甜菜碱溶液,5M,PCR级-Betaine Solution, PCR Grade
产品及特点
本产品为浓度为5M的甜菜碱的水溶液,可以直接加入到PCR反应或测序反应中提高扩增或测序的效率,其详细的用途包括:
1. 降低富含GC的模板形成二级结构的可能,有助于富含GC的模板的PCR扩增和测序。
2. 降低变性温度对碱基的依赖性,文献见Rees,W.A.,et al.,Betaine caneliminate the base pair composition dependence of DNA melting.Biochemistry 32,137-144(1993)。
3. 提高LA-PCR的扩增效率。
4. 提高T a q D N A 聚合酶的稳定性, 文献见W e i s s e n s t e i n e r , T . a n dLanchbury,J. S. Strategy for Controlling Preferential Amplificationand Avoiding False Negatives in PCR Typing. BioTechniques 21,1102-1108(1996)。
甜菜碱溶液,5M,PCR级-Betaine Solution, PCR Grade使用及效果
将本产品加入PCR反应体系中,使其终浓度在1.0-1.7 M之间即可。
99.8%盐酸胍(500g)
99.5%异硫氰酸胍(500g)
Solution I, 250ml
Solution II, 250ml
Solution III, 250ml
Neutralization Buffer, 250ml
Buffer SE, 250ml
Buffer T1, 250ml
Buffer T2, 250ml
Buffer T3, 250ml
Buffer HB, 250ml
DNA Wash Buffer Concentrate, 100 ml
DNA Wash Buffer Concentrate, 500ml
ETR Reagent, 100ml
MGC Binding Buffer Concentrate, 20ml
SPM Wash Buffer Concentrate, 40 ml
Buffer GBT, 100 ml
Endotoxin-Free Elution Buffer-100
TRK Lysis Buffer , 100ml
QVL Lysis Buffer , 100ml
Buffer ERL, 250ml
Buffer YRL, 100 ml
Buffer BRL, 100 ml
Buffer RB, 100ml
Buffer RPL, 100ml
Buffer RFL, 100ml
Buffer SP, 60ml
RNA Wash Buffer I, 100ml
RNA Wash Buffer II, 100ml
DEPC-treated Water, 100ml
Linear Acrylamide, 10ml
温馨提示:不可用于临床ZL。