酵母葡聚糖酶检测试剂盒—上海甄准
产品货号：ZZK-K-EBHLG
产品品名：酵母葡聚糖酶检测试剂盒
英文品名：Enzymatic Yeast Beta-Glucan Assay Kit
规格型号：50 assays per kit
An enzymatic procedure for the measurement of 1,3:1,6-beta-glucan inyeast. Also measures 1,3-Beta-glucan.
INTRODUCTION:
(1-3)-Beta-Glucans are widely distributed in nature, especially in algae,
fungi and yeast, but also in higher plants. They serve a variety of
biological functions. They form the major structural components of
cell walls, they act as storage carbohydrates and they sometimes play
a protective role by forming at specific sites in response to particular
stimuli such as wounding.1 Yeast Beta-glucan substantially enhances the
function of the immune system by activating macrophages, one of the
primary defences of the immune system. Literature indicates that the
potent anti-tumour properties of polysaccharide fractions extracted
from certain strains of mushrooms, in particular Grifola frondosa
also known as Maitake, can be attributed to linear 6-branched
(1-3)-Beta-glucan.2-4 Similar properties have recently been assigned to
(1-3)(1-4)-Beta-glucans from cereal grains (e.g. oats and barley).
The presence in wine of a particular high molecular weight
(~ 800 Kd) Beta-glucan, produced by the fungus, Botrytis cinerea (grey
rot) leads to clarification and filtration problems.5 Scleroglucan
(from Sclerotium rofsii), an industrial thickening agent with remarkable
thickening properties, has a very similar structure.
Megazyme offers an acid hydrolysis/enzymic procedure (K-YBGL)
for the determination of Beta-glucan in yeast and mushroom. That
method is based on acid hydrolysis to measure total Beta-glucan and
specific enzymic determination of α-glucan. Beta-Glucan is determined
by difference. A totally enzymic procedure specific for Beta-glucan has
been published by Danielson et al.6 The method employs Lyticase
from Sigma-Aldrich and exo-1,3-Beta-glucosidase/Beta-glucanase from
Megazyme. This method works well, but its widespread adoption
is limited by the high cost of Lyticase. In this booklet we describe
an alternate, simple enzymic procedure for the measurement of
(1-3)(1-6)-Beta-glucan in yeast. The method also gives quantitation
measurement of Beta-glucan in curdlan, laminarin and cereal Beta-glucan
preparations. It does not give quantitative measurement of Beta-glucan
in mushrooms, the reasons for which are currently being researched.
KITS:
Kits suitable for carrying out 50 assays are available from Megazyme.
The kits contain the full assay method plus:
Bottle 1: GlucazymeTM preparation (exo-1,3-Beta-glucanase,
endo-1,3-Beta-glucanase, Beta-glucosidase and chitinase
suspension), 2.2 mL.
Stable for 大于4 years at 4°C.
Bottle 2: GOPOD Reagent Buffer. Buffer (48 mL,
pH 7.4), p-hydroxybenzoic acid and sodium azide
(0.4% w/v).
Stable for 大于4 years at 4°C.
Bottle 3: GOPOD Reagent Enzymes. Glu cose oxidase
plus peroxidase and 4-aminoantipyrine. Freeze
dried powder.
Stable for 大于5 years at -20°C.
Bottle 4: D-Glu cose standard solution (5 mL, 1.5 mg/mL)
in 0.2% w/v benzoic acid.
Stable for 大于5 years at room temperature.
Bottle 5: Control yeast Beta-glucan preparation (~ 2 g,
Beta-glucan content stated on the bottle label).
Stable for 大于5 years at room temperature.
Bottle 6: Control starch preparation (~ 2 g, 96%
starch dwb).
Stable for 大于5 years at room temperature.
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