载体pLVX-IRES-tdTomato_详细资料

产品信息 供应商：沪震生物数量： 200 英文名： pLVX-IRES-tdTomato 保质期：半年保存条件： -20℃ 规格： 5μg pLVX-IRES-tdTomato 产品信息 产品货号产品名称产品规格优惠价 HZ0729 pLVX-IRES-tdTomato 20μl ¥请询价 使用说明 沪震质粒平台的各批次质粒菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产品的三个月内通知我司。收到质粒后请短暂离心，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。 基本信息 启动子: CMV 复制子: pUC ori 终止子: SV40 poly(A) signal 质粒分类: 病毒系列，慢病毒克隆载体 质粒大小: 8892bp 原核抗性: Amp 克隆菌株: Stbl3 培养条件: 37℃，有氧 LB 表达宿主: 哺乳细胞 诱导方式: 无须诱导，瞬时表达 5'测序引物: CMV-F：CGCAAATGGGCGGTAGGCGTG 3'测序引物: WPRE-R：CATAGCGTAAAAGGAGCAACA 质粒简介 pLVX-IRES-tdTomato is an HIV-1-based, lentiviral expression vector that allows the simultaneous expression of your protein of interest and tdTomato in virtually any mammalian cell type, including primary cells. tdTomato is a member of the family of fruit fluorescent proteins derived from the Discosoma sp. red fluorescent protein, DsRed . The vector expresses the two proteins from a bicistronic mRNA transcript, allowing tdTomato to be used as an indicator of transduction efficiency and a marker for selection by flow cytometry.Expression of the bicistronic transcript is driven by the constitutively active human cytomegalovirus immediate early promoter (PCMV IE) located just upstream of the MCS. An encephalomyocarditis virus (EMCV) internal ribosome entry site (IRES), positioned between the MCS and tdTomato, facilitates cap-independent translation of tdTomato from an internal start site at the IRES/tdTomato junction . pLVX-IRES-tdTomato contains all of the viral processing elements necessary for the production of replication-incompetent lentivirus, as well as elements to improve viral titer, transgene expression, and overall vector function. The woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) promotes RNA processing events and enhances nuclear export of viral RNA , leading to increased viral titers from packaging cells. In addition, the vector includes a Rev-response element (RRE), which further increases viral titers by enhancing the transport of unspliced viral RNA out of the nucleus . Finally, pLVX-IRES-tdTomato also contains a central polypurine tract/central termination sequence element (cPPT/CTS). During target cell infection, this element creates a central DNA flap that increases nuclear import of the viral genome, resulting in improved vector integration and more efficient transduction. The vector also contains a pUC origin of replication and an E. coli ampicillin resistance gene (Ampr ) for propagation and selection in bacteria. 质粒图谱 温馨提示：不可用于临床ZL。

产品货号	产品名称	产品规格	优惠价
HZ0729	pLVX-IRES-tdTomato	20μl	¥请询价

启动子:	CMV
复制子:	pUC ori
终止子:	SV40 poly(A) signal
质粒分类:	病毒系列，慢病毒克隆载体
质粒大小:	8892bp
原核抗性:	Amp
克隆菌株:	Stbl3
培养条件:	37℃，有氧 LB
表达宿主:	哺乳细胞
诱导方式:	无须诱导，瞬时表达
5'测序引物:	CMV-F：CGCAAATGGGCGGTAGGCGTG
3'测序引物:	WPRE-R：CATAGCGTAAAAGGAGCAACA

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