The ability of human embryonic and induced pluripotent stem cells to differentiate into all three germ layers has enormous potential for basic human developmental research and regenerative medicine. ScienCell has created a convenient multiplex PCR kit for the routine detection of human pluripotent stem cell differentiation. Multiplex PCR allows two or more genes to be amplified in a single PCR reaction by using multiple primer pairs in a single reaction mixture, allowing for considerable savings in labor, cost and precious DNA samples. All required PCR reagents are supplied in this kit. Simply add DNA template and perform the PCR reaction. Tube 1 ready-mix reaction contains NEUROD1, SMA and aFP primers that allows for the detection of ectoderm, mesoderm and endoderm, respectively