非乙酰化单克隆cAMP ELISA试剂盒

Product Highlight

• Based on the unique mouse monoclonal Anti-cAMP antibody
  It is the only EIA kit on the market with monoclonal anti-cAMP antibody (all other kits are based on polyclonal anti-cAMP antibodies). 
   
• No more hazardous chemical (acetic anhydride ) for your cAMP assay 
  The monoclonal Anti-cAMP antibody has similar affinities for non-acetylated cAMP and acetylated cAMP. Thus acetylation with the acetic anhydride is permanently eliminated from the assay.
   
• Super sensitivity and selectivity 
  The monoclonal Anti-cAMP antibody displays >10⁸ fold of selectivity over cGMP, ATP, and other nucleoside analogues. 
  The Anti-cAMP EIA Kit provides significantly improved sensitivity and selectivity over other kits based on polyclonal anti-cAMP antibodies on the market.
   
• Shortest assay time and high-throughput format 
  The Anti-cAMP EIA Kit is the best choice for drug screenings.

Product Description

Adenosine 3', 5'-cyclic monophosphate (cyclic AMP; cAMP) modulates various physiological functions such as cardiovascular biology, learning and memory, olfaction, immune response, asthma and kidney function (1,2). cAMP is produced from ATP by adenylyl cyclases and is degraded by phosphodiesterases. Stimulation of adenylyl cyclases or inhibition of phosphodiesterases can increase cellular cAMP concentrations. Blockers of adenylyl cyclase-activating receptors and inhibitors of the cAMP-specific phosphodiesterases are used for treating human diseases. For example, blocking agents for cAMP-increasing beta-adrenergic receptors (beta-blockers) are used for treating abnormal heart rhythms, high blood pressure (hypertension), myocardial infarction and heart failure. Inhibitors of cAMP specific phosphodiesterase types 2 and 4 are being tested for cognition enhancement.

Principle Outline

NewEast Biosciences cAMP ELISA Kit is a competitive immunoassay to measure the cAMP levels, either from cell extracts or from in vitro adenylyl cyclase assays. Briefly, multi-well plates are coated with goat-anti-mouse serum. cAMP in cell extracts or in in vitro adenylyl cyclase assays will competitively bind to the monoclonal anti-cAMP antibody in the presence of fixed amounts of cAMP-conjugated horse-radish peroxidase or alkaline phosphatase. Known amounts of cAMP are used as standards to generate the calculation curve. After a short incubation, the excess reagents are washed away and substrate is added. The multiwell plates are then read on a microplate reader at 450 nm or- 405 nm. The intensity of the yellow color is inversely proportional to the concentration of cAMP in samples. The measured optical density is used to calculate the concentration of cAMP in samples based on the curve from the cAMP standards.

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Publications:

1.  Heterogeneity in relaxation of different sized porcine coronary arteries to nitrovasodilators: role of PKG and MYPT1     Pflugers Arch. 2012 Feb;463(2):257-68

2.  Antiarrhythmic effect of prolonged morphine exposure is accompanied by altered myocardial adenylyl cyclase signaling in rats     Pharmacol Rep. 2012;64(2):351-9

3.  Uncoupling of M1 muscarinic receptor/G-protein interaction by amyloid beta(1-42)     Neuropharmacology. 2013 Apr;67:272-83

4.  Subendocardial Increase in Reactive Oxygen Species Production Affects Regional Contractile Function in Ischemic Heart Failure     Antioxid Redox Signal. 2013 Mar 20;18(9):1009-20

5.  Heterogeneity in relaxation of different sized porcine coronary arteries to nitrovasodilators: role of PKG and MYPT1     Pflugers Arch. 2012 Feb;463(2):257-68

6.  Structural basis of anthrax edema factor neutralization by a neutralizing antibody     Biochemical and Biophysical Research Communications Volume 417, Issue 1, 6 January 2012, Pages 324–329

7.  Transgenic rescue of defective Cd36 enhances myocardial adenylyl cyclase signaling in spontaneously hypertensive rats     Pflügers Archiv - European Journal of Physiology October 2013, Volume 465, Issue 10, pp 1477-1486

8.  Opposing HDAC4 nuclear fluxes due to phosphorylation by β adrenergic activated PKA or by activity or Epac activated CaMKII in skeletal muscle fibres     The Journal of Physiology Volume 591, Issue 14, pages 3605–3623, July 2013

9.  Sex is a major determinant of neuronal dysfunction in Neurofibromatosis Type 1     Annals of Neurology Volume 75, Issue 2, pages 309–316, February 2014

10.  cAMP–PKA inhibition of SK3 channel reduced both Ca2+ entry and cancer cell migration by regulation of SK3–Orai1 complex     Pflügers Archiv - European Journal of Physiology October 2014, Volume 466, Issue 10, pp 1921-1932

11.  Immunomodulating effects of casein-derived peptide QEPVL and QEPV on lymphocytes in vitro and in vivo     Food Funct., 2014,5, 2061-2069

12.  Agonist-Dependent And -Independent Dopamine-1-Like Receptor Signalling Differently Regulates Downstream Effectors     FEBS Journal Volume 281, Issue 21, pages 4792–4804, November 2014

13.  Alteration of vascular reactivity in heart failure: Role of phosphodiesterases type 3 and 4     British Journal of Pharmacology Volume 171, Issue 23, pages 5361–5375, December 2014

15.  Comparative study of somatostatin-human serum albumin fusion proteins and natural somatostatin on receptor binding, internalization and activation     PLoS One. 2014 Feb 27;9(2):e89932