Super Script Ⅱ阴性逆转录酶,酶

英文名称：SuperScript II Reverse Transcriptase
级别：BR
来源：Purified from E. coli expressing the pol gene of M-MLV (1,2), mutagenized to reduce the RNase H activity浓度：200u/ul
活力定义：One unit of SuperScript? II is the amount of enzyme required to incorporate 1 nmole of eoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly(A)?oligo(dT)12-18 as template?primer (3).Unit Reaction Conditions：50 mM Tris-HC1 (pH 8.3), 40 mM KC1, 6 mM MgC1 2 , 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)12-18 , 0.1 mg/m1 BSA, and enzyme in 50 ?l for 10 min. at 37℃Performance and Quality Testing：SDS-PAGE purity；endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA productEnzyme Function：RNA dependent DNA PolymeraseFinal Product Size：12.3 kb or lessNumber of Reactions：10 ReactionsOptimal Reaction Temperature：42℃Sensitivity：MediumTemplate：ssRNA；ssDNA；RNA-DNA Hybrid
产品描述：SuperScript? II Reverse Transcriptase (RT) is an improved version of SuperScript? RT. It is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. This enzyme is genetically engineered by the introduction of point mutations rather than a deletion in the RNase H active center. Like SuperScript? RT, SuperScript? II RT has reduced RNase H activity. Unlike SuperScript? RT, however, the selective mutations within the RNase H domain maintain full polymerase activity. This structural modification eliminates degradation of RNA molecules during first-strand cDNA synthesis and gives SuperScript? II RT superior performance characteristics, including：Greater first-strand cDNA yields；More full-length cDNA synthesis；Full activity at 42℃
性状：悬浮液
用途：生化研究。
保存：-20℃
Super Script Ⅱ阴性逆转录酶,酶订货即可快递送货上门，另个别产品需要冷藏运输，公司按要求进行特殊包装盒发送。