Human Wnt-1/重组人Wnt-1

Human Wnt-1/重组人Wnt-1
产品编号:100-367 产品名称:Human Wnt-1/重组人Wnt-1 产品规格:10μg
Description: Wnt-1 is a secreted protein that signals through the Frizzled family of cell surface receptors and is required for normal embryonic development. Wnt-1 activation induces a complex signaling cascade that ultimately leads to the increased expression of over fifty genes. An important component of Wnt-1 signaling is the stabilization, and resulting accumulation, of the intracellular signaling protein, beta-catenin. Wnt signaling induces and maintains the transformed phenotype and, in certain embryonic cell lines, supports self renewal in the absence of significant differentiation. Elevated levels of Wnt proteins are associated with tumorigenesis and are present in numerous human breast cancers. Mature human Wnt-1 is a glycosylated protein containing 343 amino acid residues. Recombinant human Wnt-1 is a 38.4 kDa, non-glycosylated protein containing 343 amino acid residues.
Source: E. coli
Molecular Weight: 38.4 kDa
Purity: 98 % (SDS-PAGE, HPLC)
Endotoxin level/内毒素水平: < 0.1 ng per ug of Wnt
Stabilizer: none
Formulation: lyophilized
Biological Activity: The ED50 was determined by its ability to enhance BMP-2 induced alkaline phosphatase production by murine ATDC5 cells. The expected ED50 for this effect is 1.5 - 2.5 ng/ml in the presence of 200 ng/ml of human BMP-2.
Reconstitution: Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use.
Stability: The lyophilized protein is stable for at least 2 years from date of receipt at -200C. Reconstituted Wnt-1 is stable for at least 3 months when stored in working aliquots with a carrier protein at -200C.
Avoid repeated freeze-thaw cycles.
Usage: Human Wnt-1 is offered for research use. Not for drug use. Not for human use.