重组艾滋病毒1 p24核心蛋白，Sf9来源/HIV-1 p24 Core, Sf9_详细资料

产品信息 重组艾滋病毒1 p24核心蛋白，Sf9来源/HIV-1 p24 Core, Sf9 产品编号:HIV-153产品名称:重组艾滋病毒1 p24核心蛋白，Sf9来源/HIV-1 p24 Core, Sf9产品规格:2μg/10μg/100μg Catalogue Number HIV-153 Introduction Human immunodeficiency virus (HIV) is a retrovirusthat can lead to a condition in which the immune systembegins to fail, leading to opportunistic infections. HIV primarily infects vital cells in the humanimmune systemsuch as helper T cells(specifically CD4+ T cells), macrophagesand dendritic cells. HIV infection leads to low levels of CD4+ T cells through three main mechanisms: firstly, direct viral killing of infected cells; secondly, increased rates of apoptosisin infected cells; and thirdly, killing of infected CD4+ T cells by CD8 cytotoxic lymphocytesthat recognize infected cells. When CD4+ T cell numbers decline below a critical level, cell-mediated immunityis lost, and the body becomes progressively more susceptible to opportunistic infections. HIV was classified as a member of the genus Lentivirus, part of the family of Retroviridae. Lentiviruses have many common morphologies and biological properties. Many species are infected by lentiviruses, which are characteristically responsible for long-duration illnesses with a long incubation period. Lentiviruses are transmitted as single-stranded, positive-sense, enveloped RNA viruses. Upon entry of the target cell, the viral RNA genomeis converted to double-stranded DNAby a virally encoded reverse transcriptasethat is present in the virus particle. This viral DNA is then integrated into the cellular DNA by a virally encoded integraseso that the genome can be transcribed. once the virus has infected the cell, two pathways are possible: either the virus becomes latentand the infected cell continues to function, or the virus becomes active and replicates, and a large number of virus particles are liberated that can then infect other cells. Description HIV-1 p24 Core Recombinant- derived from HIV-1 gene fragment is a 35 kDa gag polyprotein that includes all of the p24 plus additional amino acids of the C-terminus of p17 and the N-terminus of p15. The HIV-1 p24 core is glycosylated with N-linked sugars and produced using baculovirus vectors in insect cells. Purified under conditions that maintain the tetriatary structure of the p24 molecule. Source Baculovirus Insect Cells. Physical Appearance Sterile filtered colorless clear solution. Formulation The protein solution contains 10mM Tris pH-8.0, 300mM NaCl and 0.01% Tween-20. Purity Greater than 90.0% as determined by HPLC analysis & SDS-PAGE. Stability Recombinant HIV -1 p24 core sf9 although stable at 4°C for 3 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. Specificity Immunoreactive with sera from HIV infected individuals. Western blots : 0.1-1.0 µg/strip. Applications HIV-1 p24 Core antigen is suitable for ELISA and Western blots, excellent antigen for early detection of HIV seroconvertors with minimal specificity problems. Usage ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

您可能感兴趣的产品

Catalogue Number	HIV-153
Introduction	Human immunodeficiency virus (HIV) is a retrovirusthat can lead to a condition in which the immune systembegins to fail, leading to opportunistic infections. HIV primarily infects vital cells in the humanimmune systemsuch as helper T cells(specifically CD4+ T cells), macrophagesand dendritic cells. HIV infection leads to low levels of CD4+ T cells through three main mechanisms: firstly, direct viral killing of infected cells; secondly, increased rates of apoptosisin infected cells; and thirdly, killing of infected CD4+ T cells by CD8 cytotoxic lymphocytesthat recognize infected cells. When CD4+ T cell numbers decline below a critical level, cell-mediated immunityis lost, and the body becomes progressively more susceptible to opportunistic infections. HIV was classified as a member of the genus Lentivirus, part of the family of Retroviridae. Lentiviruses have many common morphologies and biological properties. Many species are infected by lentiviruses, which are characteristically responsible for long-duration illnesses with a long incubation period. Lentiviruses are transmitted as single-stranded, positive-sense, enveloped RNA viruses. Upon entry of the target cell, the viral RNA genomeis converted to double-stranded DNAby a virally encoded reverse transcriptasethat is present in the virus particle. This viral DNA is then integrated into the cellular DNA by a virally encoded integraseso that the genome can be transcribed. once the virus has infected the cell, two pathways are possible: either the virus becomes latentand the infected cell continues to function, or the virus becomes active and replicates, and a large number of virus particles are liberated that can then infect other cells.
Description	HIV-1 p24 Core Recombinant- derived from HIV-1 gene fragment is a 35 kDa gag polyprotein that includes all of the p24 plus additional amino acids of the C-terminus of p17 and the N-terminus of p15. The HIV-1 p24 core is glycosylated with N-linked sugars and produced using baculovirus vectors in insect cells. Purified under conditions that maintain the tetriatary structure of the p24 molecule.
Source	Baculovirus Insect Cells.
Physical Appearance	Sterile filtered colorless clear solution.
Formulation	The protein solution contains 10mM Tris pH-8.0, 300mM NaCl and 0.01% Tween-20.
Purity	Greater than 90.0% as determined by HPLC analysis & SDS-PAGE.
Stability	Recombinant HIV -1 p24 core sf9 although stable at 4°C for 3 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Specificity	Immunoreactive with sera from HIV infected individuals.
Western blots	: 0.1-1.0 µg/strip.
Applications	HIV-1 p24 Core antigen is suitable for ELISA and Western blots, excellent antigen for early detection of HIV seroconvertors with minimal specificity problems.
Usage	ProSpecs products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.