电磁净化试剂盒（Magnetic Purification System）

电磁净化试剂盒（Magnetic Purification System）
Innova Biosciences 公司专注于生物偶联技术（bioconjugation） – 即将两种分子结合在一起形成一个杂合结合物（hybrid conjugate）。这些标记结合物在世界各地的医院及科研机构被广泛使用。Innova 公司开发生产的“一步法”抗体标记试剂盒-Lightning-Link™系列产品极大程度的简化了结合标记流程。同时，Innova 也提供独有的结合物标记优化服务，开发更灵敏的免疫诊断检测。此外，Innova也向药物研发实验室提供一些相关产品。Innova 公司以提供突破性的技术和服务，极大的推动生命科学研究、简化试验流程作为企业的使命。这也必将使Innova 公司成为成为生命科学和生物技术行业的领导企业。
 

The AbPure™ Magnetic Purification System is a protein A-based antibody purification system with a unique set of elution and neutralization buffers.

The AbPure™ Magnetic Purification System can directly replace any existing protein A purification kit and can be used to isolate IgG antibodies from crude mixtures. A major advantage of the AbPure™ Magnetic Purification System is that the purified antibody is eluted in a buffer mixture that is compatible with all popular conjugation chemistries (e.g. Lightning-Link, InnovaCoat® GOLD, ThunderLink® PLUS and reactions involving NHS esters, carbodiimides, isothiocyanates and maleimides).

No dialysis of the antibody is needed prior to conjugation as the interfering substances found in other purification kits (e.g. Tris, citrate and glycine) have been replaced in the AbPure™ Magnetic Purification System with conjugation-friendly alternatives.

Features and Benefits

• Universal - compatible with a wide range of labeling technologies
• Quick & easy to use - less than 15 minutes hands-on time
• High levels of antibody recovery (70-90%) - save precious reagents
• No special equipment needed apart from magnetic stand - simple to use
• Flexible - perform up to 10 purifications at the smallest working scale

How does the kit work?

The method involves capturing the antibody on the Protein A Magnetic Beads. Protein A has a high affinity for the Fc regions of IgG molecules from a variety of species; once the antibody has bound to the Protein A, unwanted substances can be removed by simply collecting the beads in a magnetic stand and washing the beads . The antibody is then eluted and neutralized. View the full protocol here.