鸟苷酸酶检测试剂(GTPase assay system) Innova Biosciences 公司专注于生物偶联技术(bioconjugation) – 即将两种分子结合在一起形成一个杂合结合物(hybrid conjugate)。这些标记结合物在世界各地的医院及科研机构被广泛使用。Innova 公司开发生产的“一步法”抗体标记试剂盒-Lightning-Link™系列产品极大程度的简化了结合标记流程。同时,Innova 也提供独有的结合物标记优化服务,开发更灵敏的免疫诊断检测。此外,Innova也向药物研发实验室提供一些相关产品。Innova 公司以提供突破性的技术和服务,极大的推动生命科学研究、简化试验流程作为企业的使命。这也必将使Innova 公司成为成为生命科学和生物技术行业的领导企业。
This non radioactive colorimetric assay kit contains all the necessary reagents for measuring enzyme activity (everything included in the PiColorlock kit and more) and is ideal for high throughput drug screening.
The kits contain our PiColorlock™, a non-radioactive, superior phosphate detection reagent. Key features of PiColorLock™ include:
- Colorimetric Assay – competitor assays are radioactive.
- Special additive ensures low backgrounds with acid-labile substrates.
- Unparalleled stability of phosphate-dye complexes.
- Reagent is compatible with almost any assay buffer.
- No inhibition of color development by high concentrations of protein.
- Stable reagent formulation - long shelf life.
Colorimetric assays for GTPases are invariably based on the formation of colored complexes between an inorganic phosphate and a dye molecule under acidic conditions.
Competitor assays are beset with several problems including reagent precipitation. The high stability of PiColorLock™ ensures high stability of the colored dye-phosphate complexes (green color- see below).
资料下载:
GTPase assay protocol r9.pdf
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