产品名称: | Phospho-EGFR-T669 pAb |
同种型: | IgG |
基因ID: | 1956 |
Swiss Prot: | P00533 |
别名: | ERBB; ERBB1; HER1; mENA; NISBD2; PIG61 |
反应物种: | Human |
验证应用: | WB |
推荐稀释比: | WB 1:500 - 1:2000 |
理论分子量: | 44kDa/69kDa/77kDa/134kDa |
实际分子量: | 200kDa |
阳性样本: | A-431, HUVEC |
细胞定位: | Cell membrane,Endoplasmic reticulum membrane,Endosome,Endosome membrane,Golgi apparatus membrane,Nucleus membrane,Nucleus,Secreted,Single-pass type I membrane protein |
存储缓冲液: | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
检测图片: | | Western blot - Phospho-EGFR-T669 antibody (AP0025) Western blot analysis of extracts of A-431 and HUVEC cells, using Phospho-EGFR-T669 antibody (AP0025) at 1:1000 dilution. A431 cells were treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight. HUVEC cells were treated by EGF. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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