供应商： 泽叶生物 数量： 20 英文名： pLKO-DEST-EGFP 保质期： 三年 保存条件： -20℃低温保存
| 出品公司: | ZYbscience |
|---|---|
| 载体名称: | pLKO-DEST-EGFP |
| 质粒类型: | 慢病毒载体；RNAi载体；cDNA/shRNA gateway |
| 高拷贝/低拷贝: | 低拷贝 |
| 克隆方法: | gateway |
| 启动子: | U6 |
| 载体大小: | 8856 bp |
| 5' 测序引物及序列: | LKO.1 5' ：GACTATCATATGCTTACCGT |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素、氯霉素 |
| 筛选标记: | Hygromycin |
| 克隆菌株: | 阳性筛选用DB3.1 ，阴性筛选用OmniMAX2-T1, 重组慢病毒载体的复制用Stbl3感受态细胞，以维持质粒的稳定性。 |
| 宿主细胞（系）: | 哺乳动物细胞 |
| 备注: | 其它筛选标 |
| 产品目录号: | ZY 32684 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 慢病毒 |
LOCUS pLKO-DEST-EGFP 8856 bp DNA SYNACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors.FEATURES Location/Qualifiers source 1..8856 /organism="pLKO.DEST.EGFP" /mol_type="other DNA" misc_feature complement(114..221) /label="attR1" misc_feature 114..214 /label="attR2" CDS 241..747 /label="ORF frame 1" /translation="MGGRSRGPKLTREFSTSRQMAVFIHNFKRKGGIGGYSAGERIVD IIATDIQTKELQKQITKIQNFRVYYRDSRDPLWPRLEGVGVAPFPRLEFYRVGEALFP RQSGACALAAPLGTWRYTSGLWPRTHSTSTGRRQPAPFFGGPFAPPSTPPLVRKFPPA PQLASCRT*" misc_feature 322..337 /label="cPPT" /translation="MGGRSRGPKLTREFSTSRQMAVFIHNFKRKGGIGGYSAGERIVD IIATDIQTKELQKQITKIQNFRVYYRDSRDPLWPRLEGVGVAPFPRLEFYRVGEALFP RQSGACALAAPLGTWRYTSGLWPRTHSTSTGRRQPAPFFGGPFAPPSTPPLVRKFPPA PQLASCRT*" misc_feature complement(559..582) /label="MSCV_rev_primer" /translation="MGGRSRGPKLTREFSTSRQMAVFIHNFKRKGGIGGYSAGERIVD IIATDIQTKELQKQITKIQNFRVYYRDSRDPLWPRLEGVGVAPFPRLEFYRVGEALFP RQSGACALAAPLGTWRYTSGLWPRTHSTSTGRRQPAPFFGGPFAPPSTPPLVRKFPPA PQLASCRT*" CDS 804..1928 /label="ORF frame 3" /translation="MEAGRPLGQRPIAALLLRFLGSEAGKGWVRGRAQGRAQGRGGRP KVLRRPGILHASKAHVCRAVLLFLISGPFDLQPKLAYHDRVQAHGAPRHPRRRPQGRT HPRRRVRRLPRHAPHRRSGPNHNHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGR GRCHLRQADPEVHLHHRQAARALAHPRDHPDLRRAVLQPLPRPHEAARLLQVRHARRL RPGAHHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLQQPQR LYHGRQAEERHQGELQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPE QRPQREARSHGPAGVRDRRRDHSRHGRAVQVKRPHMHRYL*" misc_feature 956..975 /label="mPGK_F_primer" /translation="MEAGRPLGQRPIAALLLRFLGSEAGKGWVRGRAQGRAQGRGGRP KVLRRPGILHASKAHVCRAVLLFLISGPFDLQPKLAYHDRVQAHGAPRHPRRRPQGRT HPRRRVRRLPRHAPHRRSGPNHNHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGR GRCHLRQADPEVHLHHRQAARALAHPRDHPDLRRAVLQPLPRPHEAARLLQVRHARRL RPGAHHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLQQPQR LYHGRQAEERHQGELQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPE QRPQREARSHGPAGVRDRRRDHSRHGRAVQVKRPHMHRYL*" CDS 1048..1899 /label="ORF frame 1" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRTTT MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPW PTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFE GDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIED GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITL GMDELYK*" gene 1183..1896 /label="EGFP" /gene="EGFP" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRTTT MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPW PTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFE GDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIED GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITL GMDELYK*" misc_feature complement(1225..1246) /label="EGFP_N_primer" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRTTT MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPW PTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFE GDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIED GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITL GMDELYK*" misc_feature 1363..1392 /label="Y66 (EGFP)" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRTTT MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPW PTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFE GDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIED GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITL GMDELYK*" misc_feature 1833..1854 /label="EGFP_C_primer" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRTTT MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPW PTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFE GDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIED GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITL GMDELYK*" misc_feature 1972..1987 /label="cPPT" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRTTT MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPW PTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFE GDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIED GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITL GMDELYK*" misc_feature 1972..1993 /label="U3PPT" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRTTT MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPW PTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFE GDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIED GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITL GMDELYK*" misc_feature 1989..2041 /label="delta_U3" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRTTT MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPW PTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFE GDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIED GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITL GMDELYK*" misc_feature 2042..2222 /label="HIV-1_5_LTR" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRTTT MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPW PTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFE GDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIED GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITL GMDELYK*" misc_feature 2042..2222 /label="truncHIV-1_3_LTR" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRTTT MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPW PTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFE GDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIED GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITL GMDELYK*" terminator 2297..2416 /label="SV40_PA_terminator" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRTTT MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPW PTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFE GDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIED GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITL GMDELYK*" misc_feature 2385..2404 /label="EBV_rev_primer" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRTTT MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPW PTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFE GDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIED GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITL GMDELYK*" rep_origin 2434..2532 /label="SV40_origin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRTTT MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPW PTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFE GDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIED GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITL GMDELYK*" misc_feature 2517..2536 /label="SV40pro_F_primer" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRTTT MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPW PTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFE GDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIED GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITL GMDELYK*" rep_origin 2748..3054 /label="f1_origin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRTTT MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPW PTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFE GDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIED GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITL GMDELYK*" promoter 3247..3275 /label="AmpR_promoter" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRTTT MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPW PTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFE GDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIED GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITL GMDELYK*" gene 3317..4177 /label="Ampicillin" /gene="Ampicillin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRTTT MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPW PTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFE GDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIED GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITL GMDELYK*" CDS 3317..4177 /label="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*"
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