供应商： 泽叶生物 数量： 20 英文名： pTRIPZ 保质期： 三年 保存条件： -20℃低温保存
| 出品公司: | ZYbscience |
|---|---|
| 载体名称: | pTRIPZ |
| 质粒类型: | 慢病毒系统载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | TetO |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 13320bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素（Ampicillian） |
| 筛选标记: | 嘌呤霉素（Puromycin） |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型: | 诱导型 |
| 病毒/非病毒: | 慢病毒 |
LOCUS pTRIPZ 13320 bp DNA circular SYNDEFINITION pTRIPZACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors.COMMENT This file is created by Vector NTI http://www.biofeng.com/COMMENT VNTAUTHORNAME|biofeng.com|FEATURES Location/Qualifiers source 1..13320 /organism="pTRIPZ" /mol_type="other DNA" misc_feature 454..634 /label="HIV-1_5_LTR" misc_feature 454..634 /label="truncHIV-1_3_LTR" misc_feature 745..789 /label="HIV-1_psi_pack" CDS complement(1015..1476) /label="ORF frame 1" CDS 1181..2068 /label="ORF frame 2" misc_feature 1303..1536 /label="RRE" misc_feature 2067..2082 /label="cPPT" misc_feature complement(2245..2619) /label="sh_ble" gene complement(2248..2619) /label="bleo" /gene="bleo" promoter complement(2629..2647) /label="T7_promoter" CDS 2744..3700 /label="ORF frame 2" misc_feature 2963..2982 /label="pCEP_fwd_primer" misc_feature 2965..2989 /label="LNCX_primer" promoter complement(4039..4057) /label="T7_promoter" promoter 4077..5281 /label="hUbC_promoter" misc_feature complement(5294..5327) /label="loxP" misc_feature 5294..5327 /label="loxP" CDS 5334..6041 /label="ORF frame 3" misc_feature complement(6042..6075) /label="loxP" misc_feature 6042..6075 /label="loxP" misc_feature 6097..6660 /label="IRES" CDS 6673..7275 /label="ORF frame 1" gene 6688..7275 /label="puro (variant)" /gene="puro (variant)" misc_feature 7293..7868 /label="WPRE" CDS 7381..7908 /label="ORF frame 1" misc_feature 8086..8138 /label="delta_U3" misc_feature 8139..8319 /label="HIV-1_5_LTR" misc_feature 8139..8319 /label="truncHIV-1_3_LTR" misc_feature complement(8420..8437) /label="BGH_rev_primer" terminator 8423..8650 /label="bGH_PA_terminator" rep_origin 8713..9019 /label="f1_origin" misc_feature complement(9133..9153) /label="pBABE_3_primer" misc_feature complement(9139..9354) /label="SV40_enhancer" promoter 9151..9419 /label="SV40_promoter" rep_origin 9318..9395 /label="SV40_origin" misc_feature 9380..9399 /label="SV40pro_F_primer" gene 9531..10542 /label="hygroB" /gene="hygroB" CDS complement(9625..10278) /label="ORF frame 1" CDS 9874..10545 /label="ORF frame 1" terminator 10678..10797 /label="SV40_PA_terminator" misc_feature 10766..10785 /label="EBV_rev_primer" promoter complement(10841..10859) /label="M13_reverse_primer" misc_feature complement(10858..10880) /label="M13_pUC_rev_primer" promoter complement(10894..10923) /label="lac_promoter" gene complement(12006..12866) /label="Ampicillin" /gene="Ampicillin" CDS complement(12006..12866) /label="ORF frame 2" promoter complement(12908..12936) /label="AmpR_promoter" terminator 13022..13153 /label="SV40_PA_terminator" misc_feature 13110..13129 /label="EBV_rev_primer"ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac 2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag 2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa 2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag 2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt 2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat 2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag 2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg 2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac 2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg 2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt 2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc 2701 cgaggttcta gacgagttta ctccctatca gtgatagaga acgatgtcga gtttactccc 2761 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc 2821 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttatccctat cagtgataga 2881 gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt 2941 acggtgggag gcctatataa gcagagctcg tttagtgaac cgtcagatcg caccggtcgc 3001 caccatgagc gagctgatca aggagaacat gcacatgaag ctgtacatgg agggcaccgt 3061 gaacaaccac cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca 3121 gaccatgaag atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc 3181 taccagcttc atgtacggca gcaaagcctt catcaaccac acccagggca tccccgactt 3241 ctttaagcag tccttccctg agggcttcac atgggagaga atcaccacat acgaagacgg 3301 gggcgtgctg accgctaccc aggacaccag cttccagaac ggctgcatca tctacaacgt 3361 caagatcaac ggggtgaact tcccatccaa cggccctgtg atgcagaaga aaacacgcgg 3421 ctgggaggcc aacaccgaga tgctgtaccc cgctgacggc ggcctgagag gccacagcca 3481 gatggccctg aagctcgtgg gcgggggcta cctgcactgc tccttcaaga ccacatacag 3541 atccaagaaa cccgctaaga acctcaagat gcccggcttc cacttcgtgg accacagact 3601 ggaaagaatc aaggaggccg acaaagagac ctacgtcgag cagcacgaga tggctgtggc 3661 caagtactgc gacctcccta gcaaactggg gcacagataa tcgatagttt gtttgaatga 3721 ggcttcagta ctttacagaa tcgttgcctg cacatcttgg aaacacttgc tgggattact 3781 tcttcaggtt aacccaacag aaggctcgag gtaaccggat cctgatcaga attcaagggg 3841 ctactttagg agcaattatc ttgtttacta aaactgaata ccttgctatc tctttgatac 3901 atttttacaa agctgaatta aaatggtata aattaaatca cttttttcaa ttggaagact 3961 aatgcggccg gccattactc cgtctcgtgt cttgttgcat atgtctgctg gtttgtttga 4021 tgttgtttgc gggcgggccc tatagtgagt cgtattacct aggacgcgtg gcctccgcgc 4081 cgggttttgg cgcctcccgc gggcgccccc ctcctcacgg cgagcgctgc cacgtcagac 4141 gaagggcgca gcgagcgtcc tgatccttcc gcccggacgc tcaggacagc ggcccgctgc 4201 tcataagact cggccttaga accccagtat cagcagaagg acattttagg acgggacttg 4261 ggtgactcta gggcactggt tttctttcca gagagcggaa caggcgagga aaagtagtcc 4321 cttctcggcg attctgcgga gggatctccg tggggcggtg aacgccgatg attatataag 4381 gacgcgccgg gtgtggcaca gctagttccg tcgcagccgg gatttgggtc gcggttcttg 4441 tttgtggatc gctgtgatcg tcacttggtg agtagcgggc tgctgggctg gccggggctt 4501 tcgtggccgc cgggccgctc ggtgggacgg aagcgtgtgg agagaccgcc aagggctgta
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