pLVX-TRE3G-mCherry载体

LOCUS       pLVX-TRE3G-mCherry  9115 bp    DNA   circular             9-MAY-2012COMMENT     Created by Clontech Laboratories Inc.COMMENT     631360, 631349, 631352, 631255COMMENT     This file is created by Vector NTI            http://www.biofeng.com/COMMENT     VNTAUTHORNAME|biofeng.com|FEATURES             Location/Qualifiers     misc_feature    1..635                     /label=5'\LTR     misc_feature    685..822                     /label=psi     misc_feature    1303..1536                     /label=RRE     misc_feature    2028..2151                       /label=cPPT/CTS     misc_feature    3896..4399                     /label=pPGK     misc_feature    4420..5019                     /label=Puro     misc_feature    5037..5628                     /label=WPRE     3'clip          5832..6468                     /label=3'\LTR     misc_feature    complement(6938..7608)                     /label=pUC\ori     misc_feature    2564..3274                     /label=mCherry     misc_feature    3283..3871                     /label=IRES     misc_feature    3872..3895                     /label=MCS     misc_feature    2209..2560                     /label=pTRE3GV     misc_feature    complement(8614..8718)                     /label=pAmp(R)     misc_feature    complement(7753..8613)                     /label=Amp(R)BASE COUNT     2273 a      2340 c      2410 g      2092 t ORIGIN        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca        61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac       121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca       181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg       241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag       301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg       361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat       421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga       481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct       541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc       601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag       661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg       721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga       781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg       841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg       901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct       961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat      1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca      1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc      1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag      1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc      1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg      1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc      1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc      1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct      1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa      1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca      1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt      1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt      1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta      1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt      1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct      1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga      1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag      2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac      2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg      2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtt tactccctat      2221 cagtgataga gaacgtatga agagtttact ccctatcagt gatagagaac gtatgcagac      2281 tttactccct atcagtgata gagaacgtat aaggagttta ctccctatca gtgatagaga      2341 acgtatgacc agtttactcc ctatcagtga tagagaacgt atctacagtt tactccctat      2401 cagtgataga gaacgtatat ccagtttact ccctatcagt gatagagaac gtatgtcgag      2461 gtaggcgtgt acggtgggcg cctataaaag cagagctcgt ttagtgaacc gtcagatcgc      2521 ctggagcaat tccacaacac ttttgtctta tacttggatc accatggtga gcaagggcga      2581 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc      2641 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac      2701 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct      2761 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga      2821 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga      2881 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa      2941 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat      3001 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat      3061 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta      3121 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat      3181 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca      3241 ctccaccggc ggcatggacg agctgtacaa gtagggccgc cccggccggc ccctctccct      3301 cccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc      3361 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc      3421 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt      3481 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct      3541 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa      3601 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt      3661 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag      3721 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggta cacatgcttt      3781 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt      3841 ttcctttgaa aaacacgatg ataatatggc ccccgggacg cgtcatatgg aattctaccg      3901 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg      3961 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg      4021 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag      4081 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg      4141 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg      4201 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg      4261 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc      4321 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc      4381 ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta caagcccacg      4441 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc      4501 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc      4561 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg      4621 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg      4681 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa      4741 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc      4801 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga      4861 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac      4921 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga      4981 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac ggggcgcgtc tggaacaatc      5041 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt      5101 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg      5161 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc      5221 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt      5281 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg      5341 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg      5401 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct      5461 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc      5521 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc      5581 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg      5641 cagtcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc      5701 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggtttttcca gtcacacctc      5761 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag      5821 aaaagagggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt      5881 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg      5941 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg      6001 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga      6061 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc      6121 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca      6181 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc      6241 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta      6301 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa      6361 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac      6421 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt      6481 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggcct      6541 tgacattgct agcgttttac cgtcgacctc tagctagagc ttggcgtaat catggtcata      6601 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag      6661 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg      6721 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca      6781 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc      6841 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg      6901 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa      6961 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga      7021 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag      7081 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct      7141 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg      7201 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc      7261 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt      7321 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta      7381 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac      7441 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc      7501 ttgatccggc aaacaaacca ccgctggtag cggttttttt gtttgcaagc agcagattac      7561 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca      7621 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac      7681 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac      7741 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt      7801 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt      7861 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt      7921 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc      7981 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa      8041 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg      8101 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt      8161 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc      8221 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt      8281 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg      8341 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac      8401 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc      8461 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt      8521 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg      8581 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag      8641 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa      8701 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg      8761 agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt      8821 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg      8881 tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc      8941 ccaccccata ccctattacc actgccaatt acctagtggt ttcatttact ctaaacctgt      9001 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac      9061 ttagtagttt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagt