pLVX-PAmCherry-C1载体

LOCUS       pLVX-PAmCherry-C1  8770 bp    DNA   circular  SYN        8-AUG-2011COMMENT     Created by Clontech Laboratories Inc.COMMENT     Cat. No.632587COMMENT     This file is created by Vector NTI            http://www.biofeng.com/COMMENT     VNTAUTHORNAME|biofeng.com|FEATURES             Location/Qualifiers     source          1..8770                     /organism="synthetic DNA construct"                     /mol_type="other DNA"     LTR             1..635                     /label=5'\LTR                     /note="color: #000080"     misc_feature    685..822                     /label=Packaging\signal                     /note="color: #66cc99"     misc_binding    1303..1536                     /label=RRE                     /note="color: #009966"     misc_feature    2028..2151                     /label=CTS/cPPT\(DNA\flap)                     /note="color: #66cc99"     promoter        2185..2788                     /label=CMV                     /note="color: #66cc99"     CDS             2806..3513                     /label=PAmCherry                     /note="color: #ff9966"     misc_feature    3530..3594                     /label=MCS                     /note="color: #66cc99"     promoter        3596..4104                     /label=PGK\promoter                     /note="color: #66cc99"     CDS             4125..4724                     /label=Puro(R)                     /note="color: #66cc99"     misc_feature    4738..5329                     /label=WPRE                     /note="color: #66cc99"     LTR             5532..6168                     /label=3'\LTR                     /note="color: #000080"     rep_origin      complement(6638..7311)                     /label=pUC\ori                     /note="color: #ff9966"     CDS             complement(7456..8452)                     /label=Amp(R)                     /note="color: #ff9966"BASE COUNT     2178 a      2265 c      2330 g      1997 t ORIGIN        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca        61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac       121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca       181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg       241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag       301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg       361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat       421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga       481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct       541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc       601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag       661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg       721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga       781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg       841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg       901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct       961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat      1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca      1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc      1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag      1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc      1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg      1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc      1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc      1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct      1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa      1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca      1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt      1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt      1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta      1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt      1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct      1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga      1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag      2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac      2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg      2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa      2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg      2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt      2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg      2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc      2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc      2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca      2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta      2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa      2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc      2761 tccatagaag acaccgactc tagctagagg atcgctagcg ccaccatggt gagcaagggc      2821 gaggaggata acatggccat cattaaggag ttcatgcgct tcaaggtgca catggagggg      2881 tccgtgaacg gccacgtgtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc      2941 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcac ctgggacatc      3001 ctgtcccctc aattcatgta cggctccaat gcctacgtga agcaccccgc cgacatcccc      3061 gactacttta agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaaattcgag      3121 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggtga gttcatctac      3181 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc      3241 atgggctggg aggccctctc cgagcggatg taccccgagg acggcgccct gaagggcgag      3301 gtcaagccga gagtgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc      3361 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaaccg caagttggac      3421 atcacctcac acaacgagga ctacaccatc gtggaacagt acgaacgtgc cgagggccgc      3481 cactccaccg gcggcatgga cgagctgtac aaggctaccg gactcagatc tcgagctcaa      3541 gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat cccgcgactc tagataattc      3601 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg      3661 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt      3721 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta      3781 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta      3841 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc      3901 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga      3961 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg      4021 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc      4081 tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc gagtacaagc      4141 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg      4201 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg      4261 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg      4321 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg      4381 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc      4441 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg      4501 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc      4561 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc      4621 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg      4681 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaccgcgt ctggaacaat      4741 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct      4801 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg      4861 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg      4921 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt      4981 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt      5041 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg      5101 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc      5161 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat      5221 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc      5281 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattaattct      5341 gcagtcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg      5401 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc      5461 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag      5521 aaaagagggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt      5581 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg      5641 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg      5701 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga      5761 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc      5821 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca      5881 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc      5941 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta      6001 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa      6061 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac      6121 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt      6181 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggcct      6241 tgacattgct agcgttttac cgtcgacctc tagctagagc ttggcgtaat catggtcata      6301 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag      6361 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg      6421 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca      6481 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc      6541 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg      6601 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa      6661 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga      6721 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag      6781 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct      6841 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg      6901 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc      6961 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt      7021 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta      7081 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac      7141 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc      7201 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat      7261 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc      7321 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt      7381 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta      7441 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct      7501 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg      7561 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga      7621 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt      7681 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt      7741 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt      7801 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat      7861 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc      7921 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc      7981 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat      8041 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag      8101 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt      8161 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc      8221 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa      8281 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg      8341 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa      8401 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc      8461 gggagatcaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa      8521 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca      8581 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac      8641 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct      8701 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa      8761 acttagtagt //