供应商： 泽叶生物 数量： 20 英文名： pLKO.1-GFP-shRNA 保质期： 三年 保存条件： -20℃低温保存
| 出品公司: | ZYbscience |
|---|---|
| 载体名称: | pLKO.1-GFP-shRNA |
| 质粒类型: | 慢病毒载体；RNAi载体 |
| 高拷贝/低拷贝: | 低拷贝 |
| 克隆方法: | gateway |
| 启动子: | U6 |
| 载体大小: | 7091 bp |
| 5' 测序引物及序列: | LKO.1 5' ：GACTATCATATGCTTACCGT |
| 3' 测序引物及序列: | SV40pA-R ：GAAATTTGTGATGCTATTGC |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素、氯霉素 |
| 筛选标记: | Puromycin |
| 克隆菌株: | Stbl3 |
| 宿主细胞（系）: | 哺乳动物细胞 |
| 备注: | shRNA directed against GFP (Target sequence: 5'GCAAGCTGACCCTGAAGTTCAT3'). Used as control shRNA. |
| 产品目录号: | ZY30323 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 慢病毒 |
LOCUS pLKO.1 GFP shRNA Sequencing Result 7091 bp DNA SYNDEFINITION pLKO.1 GFP shRNA Sequencing ResultACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors.FEATURES Location/Qualifiers source 1..7091 /organism="pLKO.1 GFP shRNA Sequencing Result" /mol_type="other DNA" promoter 446..475 /label="lac_promoter" misc_feature 489..511 /label="M13_pUC_rev_primer" promoter 510..528 /label="M13_reverse_primer" promoter 545..564 /label="T3_promoter" misc_feature 818..998 /label="HIV-1_5_LTR" misc_feature 818..998 /label="truncHIV-1_3_LTR" misc_feature 1109..1153 /label="HIV-1_psi_pack" CDS 1541..2398 /label="ORF frame 2" /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN GSRRYRSRD*" misc_feature 1663..1896 /label="RRE" /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN GSRRYRSRD*" promoter 2432..2664 /label="hU6_promoter" /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN GSRRYRSRD*" misc_feature 2594..2613 /label="LKO1_5_primer" /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN GSRRYRSRD*" CDS 2707..3201 /label="ORF frame 1" /translation="MNFRVSLLFEFSTSRQMAVFIHNFKRKGGIGGYSAGERIVDIIA TDIQTKELQKQITKIQNFRVYYRDSRDPLWPRLEGVGVAPFPRQPWVCAGTRLLWAWF RETQRRRPWVSHILHVRSQRHPDLRRYPCGPPGDASCSAPKSGRFLAVRGVPDVTNGS RTSH*" misc_feature 2779..2794 /label="cPPT" /translation="MNFRVSLLFEFSTSRQMAVFIHNFKRKGGIGGYSAGERIVDIIA TDIQTKELQKQITKIQNFRVYYRDSRDPLWPRLEGVGVAPFPRQPWVCAGTRLLWAWF RETQRRRPWVSHILHVRSQRHPDLRRYPCGPPGDASCSAPKSGRFLAVRGVPDVTNGS RTSH*" CDS complement(2860..3474) /label="ORF frame 3" /translation="MVSSGGSPWGERSVIRSTREPTADVRSGGLQNAEHRAGRNRAHT TAPHPASRTAPSRPLLSARPAEQPLLATAHRGRRAAIAPWRCPSARVLVRRAASVCHV RHAANRKEPSRLRGGAGSVAGGPTRVAAKIRVTLRTDVKNVRDPGSAPLRFPEPRPEQ PRPCANPGLPWKRRNPNPLEPRPKWISAVPVINPKILNFCNLFL*" CDS 3233..4072 /label="ORF frame 2" /translation="MAARRPRWAVANSGCSAGRAESSGREGAVREAGCGAVVWALFLP ARCSAFCKPPERTSAVGSLVDRITDLSPQGDPPELTMTEYKPTVRLATRDDVPRAVRT LAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPE SVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKG LGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRK PGA*" CDS complement(3278..4123) /label="ORF frame 2" /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA LGAPC*" gene 3473..4072 /label="puro (variant)" /gene="puro (variant)" /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA LGAPC*" misc_feature 4183..4204 /label="U3PPT" /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA LGAPC*" misc_feature 4183..4198 /label="cPPT" /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA LGAPC*" misc_feature 4200..4252 /label="delta_U3" /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA LGAPC*" misc_feature 4253..4433 /label="HIV-1_5_LTR" /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA LGAPC*" misc_feature 4253..4433 /label="truncHIV-1_3_LTR" /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA LGAPC*" terminator 4508..4627 /label="SV40_PA_terminator" /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA LGAPC*" misc_feature 4596..4615 /label="EBV_rev_primer" /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA LGAPC*" rep_origin 4645..4743 /label="SV40_origin" /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA LGAPC*" misc_feature 4728..4747 /label="SV40pro_F_primer" /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA LGAPC*" promoter complement(4822..4840) /label="T7_promoter" /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA LGAPC*" promoter complement(4850..4866) /label="M13_forward20_primer" /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA LGAPC*" misc_feature 4859..5007 /label="lacZ_a" /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA LGAPC*" misc_feature complement(4859..4881) /label="M13_pUC_fwd_primer" /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA LGAPC*" rep_origin 5025..5331 /label="f1_origin" /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA LGAPC*" promoter 5524..5552 /label="AmpR_promoter" /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA LGAPC*" gene 5594..6454 /label="Ampicillin" /gene="Ampicillin" /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA LGAPC*" CDS 5594..6454 /label="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*"
pLKO.1-GFP-shRNA载体
![Scramble <em>shRNA</em> piggyBac对照<em>载体</em>pPB[<em>shRNA</em>]-mCherry/Puro-U6>Scramble_<em>shRNA</em>](https://item.yiqi.com/pic/CovPic/4/3329056209470037044-14.jpg)
Scramble shRNA piggyBac对照载体pPB[shRNA]-mCherry/Puro-U6>Scramble_shRNA
Scramble shRNA piggyBac对照载体pPB[shRNA]-EGFP/Neo-U6>Scramble_shRNA
Scramble shRNA AAV对照载体pAAV[shRNA]-CMV>TagBFP2-U6>Scramble_shRNA
Scramble shRNA AAV对照载体pAAV[shRNA]-CMV>mCherry-U6>Scramble_shRNA
Scramble shRNA AAV对照载体pAAV[shRNA]-CMV>EGFP-U6>Scramble_shRNA
Scramble shRNA腺病毒对照载体pAV[shRNA]-mCherry-U6>Scramble_shRNA
Scramble shRNA腺病毒对照载体pAV[shRNA]-TagBFP2-U6>Scramble_shRNA
Scramble shRNA慢病毒对照载体pLV[shRNA]-TagBFP2/hygro-U6>Scramble_shRNA
Scramble shRNA慢病毒对照载体pLV[shRNA]-mCherry/Puro-U6>Scramble_shRNA
Scramble shRNA慢病毒对照载体pLV[shRNA]-EGFP/Puro-U6>Scramble_shRNA
Scramble shRNA慢病毒对照载体pLV[shRNA]-EGFP/Neo-U6>Scramble_shRNA
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