pMT/BioEase-DEST载体_详细资料

产品信息 供应商：泽叶生物数量： 20 英文名： pMT/BioEase-DEST 保质期：三年保存条件： -20℃低温保存 载体基本信息 出品公司: ZYbscience 载体名称: pMT/BioEase-DEST 质粒类型: 昆虫表达载体；Gateway载体；分泌表达载体 克隆方法: Gateway 启动子: MT 载体大小: 5375 bp 5' 测序引物及序列: MT Forward：CATCTCAGTGCAACTAAA 3' 测序引物及序列: -- 载体标签: ioEase Tag（N-末端） 载体抗性: 氨苄青霉素、氯霉素（仅空载体） 真核筛选标记: 无 克隆菌株: DB3.1(载体复制）；TOP10；DH5-T1R 宿主细胞（系）: 果蝇细胞系S2 备注: 与pCoBlast或者pCoHygro共转染，可筛选建立稳定表达的细胞系。 pMT/BioEase-DEST是Gateway载体，需要与入门载体、供体载体一同使用。 产品目录号: ZY4140-01 瞬表达/稳表达: 瞬表达 组成型/诱导型: 诱导型（硫酸铜或氯化镉） 病毒/非病毒: 非病毒 载体质粒图谱和多克隆位点信息 载体简介 The DES BioEase Expression Kit with pCoBlast provides an easy, efficient method for expressing, purifying, and detecting biotinylated recombinant proteins. The pMT/BioEase-DEST vector incorporates a 72-amino acid sequence from K. pneumoniae that directs in vivo biotinylation of a specific lysine residue. Proteins produced in pMT/BioEase-DEST are expressed as fusion proteins to this sequence. Biotinylated fusion proteins can be purified on streptavidin agarose or detected onwestern blots using streptavidin-HRP or streptavidin-AP conjugates. In addition to the BioEase tag, the pMT/BioEase-DEST vector offers the following features: attR sites for fast and efficient recombination with any attL-flanked Gateway vectormetallothionein (MT) promoter for high level, inducible expression in S2 cells Enterokinase (EK) cleavage site for efficient removal of the N-terminal BioEasefusion tag A Blasticidin vector (pCoBlast) for rapid selection of stable cells 载体序列 LOCUS pMT/BioEase DEST 5375 bp DNA SYNDEFINITION pMT/BioEase DESTACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors.FEATURES Location/Qualifiers source 1..5375 /organism="pMT/BioEase DEST" /mol_type="other DNA" misc_feature complement(29..51) /label="pGEX_3_primer" misc_feature complement(250..393) /label="lacZ_a" misc_feature 364..386 /label="M13_pUC_fwd_primer" promoter 379..395 /label="M13_forward20_primer" misc_feature 814..831 /label="Metallothionein_primer" misc_feature 1095..1118 /label="Xpress_EK" misc_feature 1128..1252 /label="attR1" misc_feature complement(1152..1252) /label="attR2" promoter 1277..1300 /label="lac_promoter" gene 1361..2020 /label="CAT/CamR" /gene="CAT/CamR" CDS 1361..2020 /label="ORF frame 2" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" misc_feature 2341..2646 /label="ccdB" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" misc_feature complement(2687..2794) /label="attR1" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" misc_feature 2687..2787 /label="attR2" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" misc_feature complement(2858..2875) /label="BGH_rev_primer" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" terminator complement(2887..3006) /label="SV40_PA_terminator" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" misc_feature complement(2901..2920) /label="EBV_rev_primer" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" promoter complement(3151..3169) /label="M13_reverse_primer" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" misc_feature complement(3168..3190) /label="M13_pUC_rev_primer" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" promoter complement(3204..3233) /label="lac_promoter" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" gene complement(4316..5176) /label="Ampicillin" /gene="Ampicillin" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" CDS complement(4316..5176) /label="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" promoter complement(5218..5246) /label="AmpR_promoter" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*"ORIGIN 1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA 61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG 121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC 181 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC 241 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT 301 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT 361 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGCCAG TGAATTAATT CGTTGCAGGA 421 CAGGATGTGG TGCCCGATGT GACTAGCTCT TTGCTGCAGG CCGTCCTATC CTCTGGTTCC 481 GATAAGAGAC CCAGAACTCC GGCCCCCCAC CGCCCACCGC CACCCCCATA CATATGTGGT 541 ACGCAAGTAA GAGTGCCTGC GCATGCCCCA TGTGCCCCAC CAAGAGTTTT GCATCCCATA 601 CAAGTCCCCA AAGTGGAGAA CCGAACCAAT TCTTCGCGGG CAGAACAAAA GCTTCTGCAC 661 ACGTCTCCAC TCGAATTTGG AGCCGGCCGG CGTGTGCAAA AGAGGTGAAT CGAACGAAAG 721 ACCCGTGTGT AAAGCCGCGT TTCCAAAATG TATAAAACCG AGAGCATCTG GCCAATGTGC 781 ATCAGTTGTG GTCAGCAGCA AAATCAAGTG AATCATCTCA GTGCAACTAA AGGGGGGATC 841 TAGCGTTTAA ACTTAAGCTT ACCATGGGCG CCGGCACCCC GGTGACCGCC CCGCTGGCGG 901 GCACTATCTG GAAGGTGCTG GCCAGCGAAG GCCAGACGGT GGCCGCAGGC GAGGTGCTGC 961 TGATTCTGGA AGCCATGAAG ATGGAAACCG AAATCCGCGC CGCGCAGGCC GGGACCGTGC 1021 GCGGTATCGC GGTGAAAGCC GGCGACGCGG TGGCGGTCGG CGACACCCTG ATGACCCTGG 1081 CGGGCTCTGG ATCCGATCTG TACGACGATG ACGATAAGGT ACATCAAACA AGTTTGTACA 1141 AAAAAGCTGA ACGAGAAACG TAAAATGATA TAAATATCAA TATATTAAAT TAGATTTTGC 1201 ATAAAAAACA GACTACATAA TACTGTAAAA CACAACATAT CCAGTCACTA TGGCGGCCGC 1261 ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATAATGTGT GGATTTTGAG 1321 TTAGGATCCG GCGAGATTTT CAGGAGCTAA GGAAGCTAAA ATGGAGAAAA AAATCACTGG 1381 ATATACCACC GTTGATATAT CCCAATGGCA TCGTAAAGAA CATTTTGAGG CATTTCAGTC 1441 AGTTGCTCAA TGTACCTATA ACCAGACCGT TCAGCTGGAT ATTACGGCCT TTTTAAAGAC 1501 CGTAAAGAAA AATAAGCACA AGTTTTATCC GGCCTTTATT CACATTCTTG CCCGCCTGAT 1561 GAATGCTCAT CCGGAATTCC GTATGGCAAT GAAAGACGGT GAGCTGGTGA TATGGGATAG 1621 TGTTCACCCT TGTTACACCG TTTTCCATGA GCAAACTGAA ACGTTTTCAT CGCTCTGGAG 1681 TGAATACCAC GACGATTTCC GGCAGTTTCT ACACATATAT TCGCAAGATG TGGCGTGTTA 1741 CGGTGAAAAC CTGGCCTATT TCCCTAAAGG GTTTATTGAG AATATGTTTT TCGTCTCAGC 1801 CAATCCCTGG GTGAGTTTCA CCAGTTTTGA TTTAAACGTG GCCAATATGG ACAACTTCTT 1861 CGCCCCCGTT TTCACCATGG GCAAATATTA TACGCAAGGC GACAAGGTGC TGATGCCGCT 1921 GGCGATTCAG GTTCATCATG CCGTCTGTGA TGGCTTCCAT GTCGGCAGAA TGCTTAATGA 1981 ATTACAACAG TACTGCGATG AGTGGCAGGG CGGGGCGTAA ACGCGTGGAT CCGGCTTACT 2041 AAAAGCCAGA TAACAGTATG CGTATTTGCG CGCTCGCGAA CCGGTGTATA CCCGAAGTAT 2101 GTCAAAAAGA GGTGTGCTAT GAAGCAGCGT ATTACAGTGA CAGTTGACAG CGACAGCTAT 2161 CAGTTGCTCA AGGCATATAT GATGTCAATA TCTCCGGTCT GGTAAGCACA ACCATGCAGA 2221 ATGAAGCCCG TCGTCTGCGT GCCGAACGCT GGAAAGCGGA AAATCAGGAA GGGATGGCTG 2281 AGGTCGCCCG GTTTATTGAA ATGAACGGCT CTTTTGCTGA CGAGAACAGG GACTGGTGAA 2341 ATGCAGTTTA AGGTTTACAC CTATAAAAGA GAGAGCCGTT ATCGTCTGTT TGTGGATGTA 2401 CAGAGTGATA TTATTGACAC GCCCGGGCGA CGGATGGTGA TCCCCCTGGC CAGTGCACGT 2461 CTGCTGTCAG ATAAAGTCTC CCGTGAACTT TACCCGGTGG TGCATATCGG GGATGAAAGC 2521 TGGCGCATGA TGACCACCGA TATGGCCAGT GTGCCGGTCT CCGTTATCGG GGAAGAAGTG 2581 GCTGATCTCA GCCACCGCGA AAATGACATC AAAAACGCCA TTAACCTGAT GTTCTGGGGA 2641 ATATAAATGT CAGGCTCCGT TATACACAGC CAGTCTGCAG GTCGACCATA GTGACTGGAT 2701 ATGTTGTGTT TTACAGTATT ATGTAGTCTG TTTTTTATGC AAAATCTAAT TTAATATATT 2761 GATATTTATA TCATTTTACG TTTCTCGTTC AGCTTTCTTG TACAAAGTGG TGATAATTAA 2821 TTAAGATCTA GAGGGCCCGT TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAAGATC 2881 CAGACATGAT AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG CAGTGAAAAA 2941 AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA 3001 ATAAACAAGT TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT 3061 GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG TATGGCTGAT TATGATCAGT 3121 CGACCTGCAG GCATGCAAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT 3181 GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG 3241 GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT 3301 CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT 3361 TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC 3421 TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG 3481 ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG 3541 CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC 3601 GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG 3661 GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT 3721 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG 3781 TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT 3841 GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC 3901 TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT 3961 TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC 4021 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA 4081 CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT 4141 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC 4201 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT 4261 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC 4321 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG 4381 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG 4441 CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC 4501 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA 4561 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG 4621 TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT 4681 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA 4741 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG 4801 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA 4861 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT 4921 GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA 4981 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT 5041 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT 5101 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA 5161 AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT 5221 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC 5281 GCACATTTCC CCGAAAAGTG CCACCTGACG TCTAAGAAAC CATTATTATC ATGACATTAA 5341 CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGT// 温馨提示：不可用于临床ZL。

出品公司:	ZYbscience
载体名称:	pMT/BioEase-DEST
质粒类型:	昆虫表达载体；Gateway载体；分泌表达载体
克隆方法:	Gateway
启动子:	MT
载体大小:	5375 bp
5' 测序引物及序列:	MT Forward：CATCTCAGTGCAACTAAA
3' 测序引物及序列:	--
载体标签:	ioEase Tag（N-末端）
载体抗性:	氨苄青霉素、氯霉素（仅空载体）
真核筛选标记:	无
克隆菌株:	DB3.1(载体复制）；TOP10；DH5-T1R
宿主细胞（系）:	果蝇细胞系S2
备注:	与pCoBlast或者pCoHygro共转染，可筛选建立稳定表达的细胞系。 pMT/BioEase-DEST是Gateway载体，需要与入门载体、供体载体一同使用。
产品目录号:	ZY4140-01
瞬表达/稳表达:	瞬表达
组成型/诱导型:	诱导型（硫酸铜或氯化镉）
病毒/非病毒:	非病毒

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