供应商: 泽叶生物 数量: 20 英文名: pET-17b 保质期: 三年 保存条件: -20℃低温保存
出品公司: | ZYbscience |
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别名: | pET17b, pet 17b |
质粒类型: | 大肠杆菌蛋白表达 |
表达水平: | 高 |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 3306bp |
5' 测序引物: | T7 |
5' 测序引物序列: | 5'-TAATACGACTCACTATAGGG-3' |
载体标签: | N-T7 |
载体抗性: | Ampicillin |
备注: | Hosts: E.coli. Related vectors: pBR322. |
产品目录号: | ZY69663-3 |
稳定性: | 瞬时表达 Transient |
组成型: | 组成型 Constitutive |
病毒/非病毒: | 非病毒 |
The pET-17b vector (Cat. No. 69663-3) carries an N-terminal 11aa T7?Tag? sequence followed by a region of useful cloning sites. Included in the multiple cloning region are dual BstX I sites, which allow efficient cloning using an asymmetric linker. Unique sites (except for the two BstX I sites) are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circluar map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below.
LOCUS pET-17b 3306 bp DNA circular SYNDEFINITION pET-17bACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors.COMMENT This file is created by Vector NTI http://www.biofeng.com/COMMENT ORIGDB|GenBankCOMMENT VNTAUTHORNAME|biofeng.com|COMMENT VNTOAUTHORAD1|Novagen Inc.|FEATURES Location/Qualifiers source 1..3306 /organism="pET-17b" /mol_type="other DNA" terminator complement(3..131) /label="T7_terminator" misc_feature 71..89 /label="T7_Terminal_primer" misc_feature complement(237..269) /label="T7_leader" misc_feature complement(252..270) /label="Xpress_fwd_primer" misc_feature complement(278..294) /label="T7_transl_en_RBS" promoter complement(331..349) /label="T7_promoter" misc_feature 858..1049 /label="ROP" misc_feature complement(1043..1065) /label="pGEX_3_primer" gene complement(2238..3098) /label="Ampicillin" /gene="Ampicillin" CDS complement(2238..3098) /label="ORF frame 2" promoter complement(3140..3168) /label="AmpR_promoter"ORIGIN 1 ttatccggat atagttcctc ctttcagcaa aaaacccctc aagacccgtt tagaggcccc 61 aaggggttat gctagttatt gctcagcggt ggcagcagcc aactcagctt cctttcgggc 121 tttgttagca gccggatctg ctcgagcggc cgccagtgtg atggatatct gcagaattcc 181 agcacactgg cggccgttac tagtggatcc gagctcggta ccaagcttga atcccgaccc 241 atttgctgtc caccagtcat gctagccata tgtatatctc cttcttaaag ttaaacaaaa 301 ttatttctag agggaaaccg ttgtggtctc cctatagtga gtcgtattaa tttcgcggga 361 tcgagatctc gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt 421 tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac 481 gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa 541 tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca 601 ttatgttccg gatctgcatc gcaggatgct gctggctacc ctgtggaaca cctacatctg 661 tattaacgaa gcgctggcat tgaccctgag tgatttttct ctggtcccgc cgcatccata 721 ccgccagttg tttaccctca caacgttcca gtaaccgggc atgttcatca tcagtaaccc 781 gtatcgtgag catcctctct cgtttcatcg gtatcattac ccccatgaac agaaatcccc 841 cttacacgga ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta 901 tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg 961 cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc 1021 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt 1081 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg 1141 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa 1201 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atatgcggtg tgaaataccg 1261 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac 1321 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 1381 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 1441 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 1501 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 1561 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 1621 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 1681 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 1741 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 1801 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 1861 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 1921 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 1981 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 2041 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 2101 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 2161 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 2221 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 2281 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 2341 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 2401 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 2461 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 2521 gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg 2581 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 2641 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 2701 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 2761 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 2821 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc 2881 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 2941 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 3001 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 3061 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 3121 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 3181 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa 3241 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt 3301 caagaa
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