Agilent安捷伦 Pfu Turbo DNA Polymerase 高保真聚合酶 600250/600600/600255_详细资料

产品信息 供应商：恒斐生物数量：现货供应英文名： Pfu Turbo DNA Polymerase 规格： 100U/500U/1000U Agilent安捷伦 Pfu Turbo DNA Polymerase 高保真聚合酶 600250/600600/600255 First Generation Polymerases - Details & Specifications Stratagene was the first to commercialize Pfu in 1991. Since that time, many improvements have been added to native Pfu through molecular engineering. The resulting products have delivered greater performance including increased robustness, yield, fidelity, speed, length, and economy. See PfuUltra II Fusion HS DNA Polymerase or Herculase II Fusion DNA Polymerase product pages for more information about Agilent’s next generation PCR polymerases. In The Beginning There Was Native Pfu DNA Polymerase Pfu exhibits the lowest error rate of any thermostable DNA polymerase Corrects nucleotide misincorporation errors with 3’ -> 5’ exonuclease activity (proofreading) Cloned Pfu eliminates smearing and unwanted background Pfu DNA polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has been shown to exhibit superior thermostability and proofreading properties compared to other thermostable polymerases. Unlike Taq DNA polymerase, highly thermostable Pfu DNA polymerase possesses 3' -> 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. This means that Pfu DNA polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA polymerase in your PCR reactions results in blunt-ended PCR products which are ideal for cloning into blunt-ended vectors (see StrataClone). Pfu DNA polymerase is superior for techniques that require high-fidelity DNA synthesis. Cloned Pfu DNA Polymerase Available with an alternative detergent (AD) for greater economy The cloned version of Pfu allows for the manufacturing of ultrapure Pfu DNA Polymerase which virtually eliminates smearing and unwanted background artifacts while providing consistent performance and supply. Performance Equal with Economy Greater Offer Polymerases Agilent’s AD polymerases feature a reformulated buffer system that allows greater control and savings of the production costs associated with the product. The savings are then passed on to you. AD polymerases have been tested with a wide variety of targets and all perform with equal or better performance as our original polymerases with no protocol changes required. Improving upon Native Pfu with PfuTurbo DNA Polymerase Enhances overall PCR performance (yield and length) by preventing “dUTP poisoning” Provides higher yields and length than native or cloned Pfu Available in AD, hotstart, and hotstart master mix PfuTurbo DNA Polymerase, a combination of cloned Pfu and ArchaeMaxx, was the first high fidelity polymerase to overcome dUTP poisoning through the addition of ArchaeMaxx Factor. By overcoming dUTP poisoning, PfuTurbo is able to deliver higher yields and length than were previously achieved. ArchaeMaxx PCR Enhancing Factor ArchaeMaxx, developed by and exclusive to Stratagene, eliminates PCR inhibition due to the inevitable accumulation of dUTP during high fidelity PCR. dUTP accumulation is a result of dCTP deamination during PCR and inhibits archaeal proofreading DNA polymerases limiting their efficiency. ArchaeMaxx functions as a dUTPase, converting poisonous dUTP to harmless dUMP and inorganic pyrophosphate resulting in greatly enhanced overall PCR performance. Rising to New Levels with PfuUltra DNA Polymerase Provides the most accurate copies of DNA possible through PCR Includes ArchaeMaxx to increase yields and length Available in AD, hotstart, and master mix The PfuUltra DNA polymerase formulation features a genetically engineered mutant of Pfu DNA polymerase and the ArchaeMaxx polymerase-enhancing factor. Featuring an error rate three-fold lower than PfuTurbo DNA Polymerase and 18-fold lower than Taq DNA Polymerase, PfuUltra DNA Polymerase is the highest fidelity enzyme available in the world. Amplification of a 500-bp fragment using PfuUltra DNA polymerase results in errors in less than 0.5% of full-length PCR products, making PfuUltra the ideal enzyme when accurate copies are your primary concern. A New Generation of Pfu-Based Polymerase Reduces PCR run times Increases potential amplicon length Increases template integrity by minimal exposure to cycling temperatures We have dramatically increased the processivity of Pfu-based enzymes by fusing our PfuUltra and Herculase DNA polymerases with a high affinity double-stranded DNA binding domain. This domain serves to better anchor the DNA polymerase, preventing early dissociation from the DNA template. The improved processivity allows the enzymes to incorporate more nucleotides per binding event, enhancing PCR yields and requiring shorter PCR extension times, hence saving you time and increasing throughput. To learn more about our fusion technology, see related links. PfuUltra II Fusion HS DNA Polymerase Dramatically reduced extension times allow for faster cycling, higher throughput, and improved template integrity. The industry standard for ultra high fidelity PCR providing the most accurate copies of DNA. Up to 19kb target length capability with little to no optimization. Includes ArchaeMaxx to increase yields and length. Herculase II Fusion DNA Polymerase Economical enough for routine high fidelity PCR Delivers the highest yields of any polymerase across a broad range of targets Amplification of difficult targets such as GC-rich or samples with inhibitors present Fast cycling with reduced extension times due to fusion technology Up to 12kb target length capability without significant optimization (23kb with optimized cycling conditions) Includes ArchaeMaxx to increase yields and length Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT) is an RNA-dependent DNA polymerase. It is a monomeric protein encoded by the central portion of the MMLV polymerase gene. The enzyme expresses both reverse transcriptase (RT) and RNase H activities. Unit Recommendation Moloney Murine Leukemia Virus Reverse Transcriptase has been cloned by several laboratories and varies in the unit per microgram (U/µg) of template recommendation. This may be due to the nonstandardized unit determinations or the inherent differences in the cloned constructs. Stratagene’s Moloney Murine Leukemia Virus Reverse Transcriptase has been tested thoroughly with RNA templates ranging from 0.24 to 9.0 kb. For targets up to 6kb, 50U has been optimized to produce full-length clones without detectable hairpin structures on 1-5 µg of template. For targets larger than 6kb, 100U is recommended. 温馨提示：不可用于临床ZL。

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