人HIF1信号应答定量PCR芯片试剂盒

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美国ScienCell研究实验室（www.sciencellonline.com）成立于1999年，公司总部位于美国加州的亚哥。主要致力
于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养
基的研究和开发，在拥有众多客户。在国内销售15年来，很多老师应用其产品发表了高质量的SCI文章，且有着极高
的文献引用率。凭借着严格的质控和优秀的产品品质，深受广大科研工作者的信赖。

ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制，细胞纯度可达98％。其中包括21
种人体正常细胞系统，90多种不同细胞类型。大多数细胞在唯有ScienCell实验室能够成功分离，产品质量过硬。
确保了实验结果的重复性和连贯性。

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人HIF1信号应答定量PCR芯片试剂盒描述：

 

ScienCell's GeneQuery™ Human HIF1 Signaling Response qPCR Array Kit (GQH-HFT) is designed to facilitate gene expression profiling of 88 key genes involved in HIF1 downstream signaling upon hypoxia-induced activation.  HIF1 activity is a major component of the human response to hypoxia.  Brief examples of how included HIF1 downstream genes may be grouped according to regulatory function are shown below:

• Cell cycling: DHFR, MAP3K5, POLE2, PRC1, SKP2
• Extracellular matrix remodeling: COL1A1, COL5A1, LOX, P4HA1, PLOD1
• Angiogenesis: HMOX1, MMP2, VEGFA, ANGPTL4, PGF
• Metabolism: PKM, STC2, DHX35, DDX11, ERO1A

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

GeneQuery™ Human HIF1 Signaling Response qPCR Array Plate Layout* (8 controls in Bold and Italic)

*gene selection may be updated based on new research and development