人皮肤伤口愈合定量PCR芯片试剂盒

Sciencell公司产品由上海中乔新舟生物科技有限公司优质供应  

美国ScienCell研究实验室（www.sciencellonline.com）成立于1999年，公司总部位于美国加州的亚哥。主要致力
于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养
基的研究和开发，在拥有众多客户。在国内销售15年来，很多老师应用其产品发表了高质量的SCI文章，且有着极高
的文献引用率。凭借着严格的质控和优秀的产品品质，深受广大科研工作者的信赖。

ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制，细胞纯度可达98％。其中包括21
种人体正常细胞系统，90多种不同细胞类型。大多数细胞在唯有ScienCell实验室能够成功分离，产品质量过硬。
确保了实验结果的重复性和连贯性。

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人肝癌定量PCR芯片试剂盒描述：


 

ScienCell's GeneQuery™ Human Hepatocellular Carcinoma qPCR Array Kit (GQH-HCC) is designed to facilitate gene expression profiling of 88 key genes involved in hepatocellular carcinoma tumorigenesis. Hepatocellular carcinoma is the most common type of liver cancer. It tends to occur in livers damaged by chronic infections of hepatitis B and/or C, alcohol abuse, type II diabetes and cirrhosis.  Brief examples of how included genes may be grouped are shown below:

• Chronic hepatitis B virus and hepatitis C virus infection response: AKT1, NFKB1, SOCS3, TGFB1
• DNA damage response and apoptosis: CASP8, MSH2, BAX, BCL2, BIRC5, FAS, TP53
• Cell cycling: CCND1, CCND2, CDKN1A, CDKN2A, BIRC5, MYC, PTEN, RASSF1
• Major signaling pathways involved in HCC tumorigenesis
  • Wnt/β-catenin: AXIN1, CCND1, CCND2, CTNNB1, EP300, FZD7, LEF1, RHOA, SFRP2, SMAD4
  • VEGF: VEGFA, AKT1, KDR, PTGS2, PTK2, RAC1, NRAS
  • FGF: AKT1, BAX, NFKB1, RAC1
  • MAPK: EGF, FAS, GADD45B, PDGFRA, TGFB1, TGFBR2, RAC1
  • PI3K: BCL2, FLT1, CREB3L3, IRS1, ITGB1, PTK1, MCL1, PTEN, RELN
• Other genes with demonstrated implications in HCC development: IGF2R, PDGFRL, MET, GLUL, MTUS1, FGL1, PEG10, MAGEC2, STARD13, FAH, GPC3

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Hepatocellular Carcinoma qPCR Array Plate Layout*

(8 controls in Bold and Italic)

* gene selection may be updated based on new research and development