CAS号:
细胞分离和扩增
供应商:
恒斐生物
数量:
现货
英文名:
Dynabeads® Untouched™ Human NK Cells Kit
保存条件:
Store at 2-8°C
规格:
1 kit
描述Use this kit to isolate pure and viable untouched NK cells from PBMC by negative isolation. The kit depletes T cells, B cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human NK cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free NK cells are truly untouched, and in perfect shape for any functional assay and application.
• High purity, recovery and viability of human untouched NK cells • Easy to use and to scale up • No columns required
Methodology: An antibody mix towards the non-NK cells is added to the sample and allowed to bind to the cells. Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human NK cells can be directly analyzed in a flow cytometer and used in any application.
规格
Form:
Bead
Capacity:
Processes ~1x10^9 cells⁄kit
Cell Type:
NK Cells
Product Size:
1 kit
Final Product:
Cells
Target Species:
Human
Isolation Method:
Negative Isolation
Output Viability:
>99%
Number of Reactions:
20 Reactions
Sample Type (Specific):
MNC
Starting Material (Cell #):
~5x10^7 cells/test
Shipping Condition:
Room Temperature
内容及储存
The Kit Contains: 10 ml Depletion MyOne™ SA Dynabeads® and 2 ml Antibody Mix with biotinylated monoclonal antibodies towards human CD3, CD14, CD36, HLA Class II, CD123 and CD235a (Glycophorin A). Store at 2-8°C
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