干细胞信号转导PCR基因芯片 Stem Cell Signaling PCR Array

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Stem Cell Signaling PCR Array

干细胞信号转导PCR基因芯片
 

Product	Species	Technology	Cat. No.
Stem Cell Signaling PCR Array	Human	Gene Expression	PAHS-047Z
Stem Cell Signaling PCR Array	Mouse	Gene Expression	PAMM-047Z
Stem Cell Signaling PCR Array	Rat	Gene Expression	PARN-047Z

    Stem Cell Signaling RT² Profiler™ PCR Array profiles the expression of 84 key genes involved in signal transduction pathways important for embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) maintenance and differentiation. A variety of growth factors maintains pluripotent status and directs differentiation of ESC and iPSC cells. If an initial stem cell line lacks the corresponding signaling effectors recognizing those growth factors, precious time and resources would be wasted attempting to differentiate unresponsive cells. Therefore, evaluating the expression of signaling genes in pluripotent and multipotent stem cells helps researchers screen clones for the presence of the differentiation signaling machinery. The array represents the receptors and transcription factors of the major signaling pathways involved in pluripotent cell maintenance and differentiation, including Fibroblast Growth Factor, Hedgehog, Notch, TGF? and WNT. Monitoring the expression of receptors and co-receptors insures that stem cells can recognize the necessary growth factors or other receptor ligands. Monitoring the expression of transcription factors and co-factors insures that the activated signaling pathway can successfully regulate gene transcription for the desired differentiation program. Using real-time PCR, you can easily and reliably analyze the expression of a focused panel of signaling genes involved in ESC and iPSC maintenance and differentiation with this array.
    干细胞信号转导PCR基因芯片可以同时检测参与胚胎干细胞（ESC）及诱导多能干细胞（IPSC）维护和分化的信号转导通路的84个关键基因的表达。多种生长因子共同维持干细胞的多能性状态，并指示ESC和IPSC细胞的分化。在干细胞研究中，如果初始的干细胞缺乏对这些生长因子的识别和反应，研究者将浪费大量宝贵的时间和资源来区分反应迟钝的细胞。因此，评估多能干细胞相关基因的表达有助于研究者筛选合适的克隆，并研究其分化的分子机制。该芯片包含参与多能干细胞维持和分化的主要信号转导通路的受体和转录因子，包括成纤维细胞生长因子（bFGF）、Hedgehog、Notch、TGF和WNT。对这些受体和共受体表达的监测，可以确保干细胞能够识别所需的生长因子或其他受体的配体。对已激活的转录因子和辅助因子表达的监测，可确保干细胞能够成功地调节相关基因的转录并满足其诱导的分化过程。利用实时定量PCR，研究者可以方便并且可信地对与干细胞维护和分化相关的基因进行同时检测。
Pluripotency Maintenance Pathway:
Receptors:IL6ST (GP130), LIFR.
Transcription Factor:STAT3.
Fibroblast Growth Factor (FGF) Signaling Pathway:
Receptors:FGFR1, FGFR2, FGFR3, FGFR4.
Transcription Factor:CDX2.
Hedgehog Signaling Pathway:
Receptors & Co-Receptors:PTCH1, PTCHD2, SMO.
Transcription Factors & Co-Factors:GLI1, GLI2, GLI3, SUFU.
Notch Signaling Pathway:
Receptors & Co-Receptors:NCSTN, NOTCH1, NOTCH2, NOTCH3, NOTCH4, PSENEN, PSEN1, PSEN2.
Transcription Factor:RBPJL.
TGFβ Superfamily Signaling Pathway:
Receptors & Co-Receptors:ACVRL1, ACVR1, ACVR1B, ACVR1C, ACVR2A, ACVR2B, AMHR2, BMPR1A, BMPR1B, BMPR2, ENG, LTBP1, LTBP2, LTBP3, LTBP4, RGMA, TGFBR1, TGFBR2, TGFBR3, TGFBRAP1.
Transcription Factors & Co-Factors:EP300, SMAD1, SMAD2, SMAD3, SMAD4, SMAD5, SMAD6, SMAD7, SMAD9, CREBBP, E2F5, RBL1, RBL2, SP1, ZEB2.
Wnt Signaling Pathway:
Receptors:FZD1, FZD2, FZD3, FZD4, FZD5, FZD6, FZD7, FZD8, FZD9, LRP5, LRP6, VANGL2.
Transcription Factors & Co-Factors:BCL9, BCL9L, CTNNB1, LEF1, NFAT5, NFATC1, NFATC2, NFATC3, NFATC4, PYGO2, TCF7L1, TCF7L2, TCF7.