干细胞miRNA的PCR芯片 Stem Cell miScript miRNA PCR Array

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Stem Cell miScript miRNA PCR Array

干细胞miRNA的PCR芯片
 

Product	Species	Technology	Cat. No.
Stem Cell miScript miRNA PCR Array	Human	miRNA Expression	MIHS-122Z
Stem Cell miScript miRNA PCR Array	Mouse	miRNA Expression	MIMM-122Z
Stem Cell miScript miRNA PCR Array	Rat	miRNA Expression	MIRN-122Z

The Human Stem Cell miScript miRNA PCR Array profiles the expression of 84 miRNAs relevant to the induction, maintenance, differentiation, and identification of stem cells. All stem cells are capable of both self-renewal and differentiation into multiple cell types. Pluripotent stem cells that are capable of differentiating into any of the cell types from any of the 3 germ layers express a common set of miRNAs that are critical to pluripotency, including some that can enhance the reprogramming of differentiated cells back to a stem cell state. However, recent research suggests that both induced pluripotent stem cells (iPSC) and primed (or epiblast) stem cells (epiSC) have different miRNA expression profiles than embryonic stem cells (ESC). During differentiation, the expression levels of pluripotency markers decline while other miRNAs critical for differentiation increase in expression. Additional miRNA become more prevalent at specific stages or in specific lineages, resulting in unique patterns of miRNA expression for progenitor cells from the 3 different germ layers. This array determines the relative expression of miRNA important for the pluripotency and differentiation of stem cells. Additional markers indicative of embryonic stem cells (ESC), induced pluripotent stem cells (iPSC), epiblast stem cells (epiSC), embryoid body cells, and germ layer progenitor cells are also represented. A set of controls present on each array enables data analysis using the ΔΔCT method of relative quantification, assessment of reverse transcription performance, and assessment of PCR performance. Using SYBR Green real-time PCR, the expression of a focused panel of miRNAs related to stem cells can be easily and reliably analyzed with this miScript miRNA PCR Array.
人类干细胞miRNA的PCR芯片用于研究干细胞感应、维护、分化和识别相关的84 个miRNA的表达。所有的干细胞都能够自我更新和分化成多种细胞类型。多能干细胞能够分化成3胚层的任何类型细胞，而3胚层表达一组对多能性至关重要的miRNA，包括可以提高重编程使分化细胞回到干细胞状态。然而,最近的研究表明, 与胚胎干细胞(ESC)相比,诱导多能干细胞(iPSC)和始发态（或外胚层）干细胞(epiSC)的miRNA表达有差异。在分化过程,多能性标记物的表达水平下降而其他分化关键miRNA的表达增加。附加的miRNA在特定阶段或在特定血统变得更加普遍,导致miRNA对三种不同胚层祖细胞独特的表达模式。这个芯片确定miRNA的相对表达对多能性和分化干细胞重要性。芯片还额外包括代表胚胎干细胞(ESC),诱导多能干细胞(iPSC),外胚层干细胞(epiSC),胚状体的身体细胞,胚层祖细胞的标记。每张芯片含一个对照组使得分析数据时可以用相对定量ΔΔCT方法评估逆转录效率,基因组DNA污染,和PCR效率。利用这张芯片，通过实时定量PCR，可以简易且可靠地检测与干细胞最相关的miRNA的表达。
Pluripotency Markers:miR-302a-3p, miR-302a-5p, miR-302b-3p, miR-302b-5p, miR-302c-3p, miR-302c-5p, miR-302d-3p, miR-367-3p, miR-371a-5p, miR-372-3p, miR-373-3p, miR-373-5p.
Pluripotency Reprogramming Enhancers:miR-302a-3p, miR-302a-5p, miR-302b-3p, miR-302b-5p, miR-302c-3p, miR-302c-5p, miR-302d-3p, miR-367-3p.
Embryonic Stem Cell (ESC) Markers:miR-182-5p, miR-222-3p, miR-517a-3p, miR-518b, miR-518c-3p, miR-520b, miR-520e, miR-520g-3p.
Induced Pluripotent Stem Cell (iPSC) Markers:miR-106b-5p, miR-125b-5p, miR-136-5p, miR-141-3p, miR-142-3p, miR-18a-5p, miR-181b-5p, miR-196a-5p, miR-196b-5p, miR-199b-3p, miR-19a-3p, miR-20a-5p, miR-214-3p, miR-221-3p, miR-24-2-5p, miR-24-3p, miR-25-3p, miR-29a-3p, miR-30e-5p, miR-323a-3p, miR-410-3p, miR-495-3p, miR-517a-3p, miR-518b, miR-518c-3p, miR-92a-3p.
Epiblast or Primed Stem Cell (epiSC) Markers:miR-135a-5p, miR-141-3p, miR-181b-5p, miR-200a-3p, miR-200b-3p, miR-218-5p, miR-375, miR-429-3p, miR-487b-3p, miR-489-3p, miR-520a-3p, miR-539-5p, miR-665, miR-96-5p.
Differentiation Factors:let-7a-5p, let-7b-5p, let-7c-5p, let-7d-5p, let-7e-5p, let-7f-5p, let-7g-5p, let-7i-5p, miR-106b-5p, miR-17-3p, miR-17-5p, miR-200a-3p, miR-200b-3p, miR-200c-3p, miR-20a-5p, miR-93-5p, miR-96-5p.
Embryoid Body Markers:miR-323a-3p, miR-487b-3p, miR-489-3p, miR-539-5p, miR-665.
Germ Layer Differentiation:
Ectodermal Progenitor Cell Markers:miR-134-5p, miR-135b-5p, miR-18a-5p, miR-18b-5p, miR-200a-3p, miR-200b-3p, miR-200c-3p, miR-21-5p, miR-214-3p, miR-323a-3p, miR-376a-3p, miR-424-5p, miR-495-3p, miR-96-5p, miR-98-5p.
Endodermal Progenitor Cell Markers:miR-10a-5p, miR-103a-3p, miR-122-5p, miR-136-5p, miR-141-3p, miR-17-3p, miR-17-5p, miR-192-5p, miR-196a-5p, miR-196b-5p, miR-206, miR-218-5p, miR-224-5p, miR-24-3p, miR-326, miR-335-5p, miR-375, miR-93-5p.
Mesodermal Progenitor Cell Markers:miR-125b-5p, miR-141-3p, miR-145-5p, miR-17-3p, miR-17-5p, miR-18a-5p, miR-18b-5p, miR-202-3p, miR-335-5p, miR-93-5p, miR-98-5p.
Mesenchymal Stem Cells Markers:miR-10a-5p, miR-145-5p, miR-181b-5p, miR-196a-5p, miR-196b-5p, miR-21-5p, miR-214-3p, miR-218-5p, miR-221-3p, miR-222-3p, miR-29a-3p, miR-424-5p, miR-98-5p.

实验原理：

What miScript miRNA PCR Arrays offer?
   Guaranteed Performance* - ready-to-use for gene expression analysis
   Time & Cost Saving - less than 30 min hands-on time to analyze 84 or 372 miRNA
   Simple Data Analysis - easy-to-use Excel-Based Data Analysis Template