信号转导通路搜寻PCR基因芯片Signal Transduction Pathway Finder PCR Array

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Signal Transduction Pathway Finder

PCR Array

信号转导通路搜寻PCR基因芯片
 

Product	Species	Technology	Cat. No.
Signal Transduction PathwayFinder PCR Array	Human	Gene Expression	PAHS-014Z
Signal Transduction PathwayFinder PCR Array	Mouse	Gene Expression	PAMM-014Z
Signal Transduction PathwayFinder PCR Array	Rat	Gene Expression	PARN-014Z
Signal Transduction PathwayFinder PCR Array	Fruit Fly	Gene Expression	PADM-014Z
Signal Transduction PathwayFinder PCR Array	Zebrafish	Gene Expression	PAZF-014Z

Transduction PathwayFinder RT² Profiler PCR Array profiles the expression of 84 key genes responsive to signal transduction pathway activation or inhibition. Cellular signaling forms a complex network of gene interactions involving multiple signal transduction pathways. Each pathway ultimately increases or decreases the expression of its target genes resulting in alteration of cellular processes. Changes in target gene expression suggest signaling pathway activation or inhibition. However, gene expression results in the same pathway vary widely, depending on model systems and experimental conditions. Therefore, multiple target genes from each pathway should be examined to ensure accurate signaling pathway identification in a variety of model systems. In addition, analyzing multiple pathways simultaneously tests for signaling pathway crosstalk. This array includes target genes for 10 commonly studied signal transduction pathways, including pathways important for developmental, immunological, metabolic, and stress-activated processes. Results obtained with this array can suggest pathways that are potentially activated or inhibited by an experimental stimulus for further follow-up studies. Using real-time PCR, research studies can easily and reliably analyze the expression of a focused panel of genes involved in cellular signaling with this array.
信号转导通路搜寻PCR基因芯片可以同时检测响应信号转导通路激活或YZ的84个关键基因的表达。细胞信号是一个复杂的，涉及多个信号转导通路的相互作用网络。每个通路ZZ增加或减少，会改变靶基因的表达。靶基因表达的激活或YZ可能来源于其相关信号通路的变化。然而，同一个信号通路的基因在不同模型系统和实验条件下表达变化非常大。因此，对各通路的多个靶基因在特定模型系统中进行检查，可以准确识别可能参与的信号转导通路。此外，同时分析多种信号传导通路可以有效研究通路之间的相互调控。该芯片包括10个常用的信号转导研究通路，包括发育，免疫，代谢的重要途径，及应激活化过程中的靶基因。芯片的结果可以有效揭示可能激活或YZ的相关通路，为后续研究提供方向。利用实时定量PCR，研究者可以方便并且可信地对细胞信号传导通路相关基因进行同时检测。
TGFß Pathway:ATF4, CDKN1B (p27KIP1), EMP1, GADD45B, HERPUD1, IFRD1, MYC, TNFSF10 (TRAIL).
WNT Pathway:AXIN2, CCND1, CCND2, DAB2, FOSL1 (FRA-1), MMP7 (Matrilysin), MYC, PPARD, WISP1.
NFκB Pathway:BCL2A1 (Bfl-1/A1), BCL2L1 (BCL-X), BIRC3 (c-IAP1), CCL5 (RANTES), CSF1 (MCSF), ICAM1, IFNG, STAT1, TNF.
JAK/STAT Pathway:
JAK1, 2 / STAT1:IRF1.
STAT3:BCL2L1 (BCL-X), CCND1, CEBPD, LRG1, MCL1, SOCS3.
STAT5:BCL2L1 (BCL-X), CCND1, SOCS3.
JAK1, 3 / STAT6:FCER2, GATA3.
p53 Pathway:BAX, BBC3, BTG2, CDKN1A (p21CIP1/WAF1), EGFR, FAS (TNFRSF6), GADD45A, PCNA, RB1.
Notch Pathway:HES1, HES5, HEY1, HEY2, HEYL, ID1, JAG1, LFNG, NOTCH1.
Hedgehog Pathway:BCL2, BMP2, BMP4, PTCH1, WNT1, WNT2B, WNT3A, WNT5A, WNT6.
PPAR Pathway:ACSL3, ACSL4, ACSL5, CPT2, FABP1, OLR1, SLC27A4, SORBS1.
Oxidative Stress:FTH1, GCLC, GCLM, GSR, HMOX1, NQO1, SQSTM1, TXN, TXNRD1.
Hypoxia:ADM, ARNT, CA9, EPO, HMOX1, LDHA, SERPINE1 (PAI-1), SLC2A1, VEGFA.