蛋白酶激活受体信号转导PCR基因芯片 Protease Activated Receptor Signaling PCR Array

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Protease Activated Receptor Signaling

PCR Array

蛋白酶激活受体信号转导PCR基因芯片
 

Product	Species	Technology	Cat. No.
Protease Activated Receptor Signaling PCR Array	Human	Gene Expression	PAHS-159Z
Protease Activated Receptor Signaling PCR Array	Mouse	Gene Expression	PAMM-159Z
Protease Activated Receptor Signaling PCR Array	Rat	Gene Expression	PARN-159Z

Protease-Activated Receptor Signaling RT² Profiler PCR Array profiles the expression of 84 key genes involved in the activation and response of protease-activated receptors (PARs). The PAR family is a class of G protein-coupled receptors that are activated by proteolytic cleavage of their extracellular domain. Thrombin (F2) activates PAR1, PAR2, and PAR4, whereas trypsin activates PAR3. However, these 4 receptors can also be activated by several other proteases. Each enzyme cleaves specific sites on the receptors, resulting in different downstream responses. The majority of the proteases that activate PAR signaling play a central role in hemostasis, or the formation and degradation of blood clots. Specific PAR signaling pathways and responses have been identified for some of these proteases, such as tissue factor (F3), activated protein C (PROC), factor VIIa (F7), and factor Xa (F10). PAR signaling also cross-talks with other cellular receptors, such as EPCR (PROCR), TLR4, and S1PR3. These signaling pathways have been identified in multiple cell types, affecting biological processes such as adhesion, proliferation, and migration. PAR signaling dysregulation can be involved in cancer progression. In addition, cancer patients are often diagnosed with coagulopathies, caused by dysregulation of either PAR ligands or target genes involved in hemostasis. PAR signaling target genes also include cytokines and other proteins regulating the inflammatory response, as well as angiogenic genes. This array includes ligands and receptors involved in PAR signaling, as well as downstream effectors and target genes identified for specific PAR signaling pathways. The results of this array can suggest which PARs and specific pathways are involved in a model system of interest. Using real-time PCR, research studies can easily and reliably analyze the expression of a focused panel of genes involved in PAR signaling with this array.
蛋白酶激活受体信号转导PCR基因芯片可以同时检测与活化及响应蛋白酶激活受体（PARS）相关的84个关键基因的表达。PAR家族是一类G蛋白偶联型受体，其胞外结构域被蛋白水解酶切割可以激活受体。凝血酶（F2）激活PAR1，PAR2，PAR4，而胰蛋白酶激活PAR3。然而，这4个受体也可以由几个其他的蛋白酶被激活。不同的酶切割受体上特定的位点，会引起不同的下游反应。大多数蛋白酶激活PAR的活性在止血，或血液凝块的形成和降解中发挥核心作用。一些具体的PAR的信号转导途径和响应受不同蛋白酶调控，已明确的包括组织因子（F3），活化蛋白C（PROC），凝血因子Ⅶa（F7），和因子Xa（F10）。PAR信号也参与其他细胞的受体的调控，如EPCR（PROCR），TLR4，S1PR3。这些信号转导通路已确定在多种细胞类型中影响生物过程，如黏附，增殖和迁移。PAR信号失调可以参与癌症的发展。此外，癌症患者通常诊断出同时患有凝血功能障碍，这是PAR配体本身或参与止血的靶基因失调所造成的。PAR信号靶基因还包括细胞因子和其他调节炎症反应的蛋白质，以及血管生成基因。该芯片包括涉及在PAR通路中的配体和受体，以及已确定的特定的下游效应和靶基因。芯片结果可以在特定的生物模型中研究PAR具体途径所涉及的相关基因。利用实时定量PCR，研究者可以方便并且可信地对PAR信号通路相关基因进行同时检测。
Protease Activated Receptors (PARs):F2R (PAR1), F2RL1 (PAR2), F2RL2 (PAR3), F2RL3 (PAR4).
Proteases Activating PARs:CTSG, F10, F2 (Thrombin), F3 (Tissue Factor), F7, MMP1, PLG, PROC (Protein C).
Thrombin Signaling:
Ligands & Receptors:F2 (Thrombin), F2R (PAR1), F2RL1 (PAR2), F2RL2 (PAR3), F2RL3 (PAR4).
Downstream Signaling:ADCY7, AKT1, GNAI1, HRAS, IKBKB, MAP2K1 (MEK1), MAPK14, PRKCA, PRKCE, PTK2, RHOA, RHOH, RPS6KB1, SRC.
Transcription Factors:EGR1, ELK1, GATA2, NFKB1, REL, RELA.
Adhesion:ICAM1, ITGAM, ITGB1, KITLG, RHOA, RHOH, S1PR1, SELE, SELP, SRC, THBS1, VCAM1.
Coagulation:F10, F2 (Thrombin), F2R (PAR1), F2RL1 (PAR2), F2RL2 (PAR3), F2RL3 (PAR4), F3 (Tissue Factor), F7, HSPA5 (GRP78), MMP1, PLAU (uPA), PLAUR (uPAR), PLEK, PLG, PROC (Protein C), PROCR (EPCR), SELP, SERPINB2 (PAI-2), SERPINE1 (PAI-1), TFPI, THBD, THBS1.
Regulated Genes:
Activated Protein C Signaling: CD55, DKK1, ETS1, F3, HBEGF (DTR), IL6, IL8, JUNB, NFATC1, PLAUR (uPAR), PTGS2 (COX2), PTPN11, RELA, SERPINB2 (PAI-2), THBS1, TP53, VEGFA.
Thrombin Signaling: CCL2 (MCP-1), CD55, DKK1, ETS1, F3, HBEGF (DTR), HMGCR, IL1B, IL6, IL8, JUNB, KDR, KITLG, MIF, NAB2, NFATC1, PLAUR (uPAR), PTGS2 (COX2), PTPN11, SERPINB2 (PAI-2), S1PR3, THBS1, TNF, VEGFA.
Factor VIIa/PAR1/2 Signaling:CDKN1A (p21CIP1/WAF1), CSF2 (GM-CSF), CTGF, NFKB1, PLAU (uPA), SERPINE1 (PAI-1).
Factor VIIa/PAR2 Signaling:CSF1 (MCSF), CSF2 (GM-CSF), CXCL1, CYR61, ITGB1, VEGFC.
TLR4/PAR2:IL10, IL13, IL4, IL6, TNF.
Other Targets:GJA1, MMP2, SELE, SELP, SERPINB5 (Maspin), TGFB1, VCAM1.