原纤毛PCR基因芯片 Primary Cilia PCR Array

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Primary Cilia PCR Array

原纤毛PCR基因芯片
 

Product	Species	Technology	Cat. No.
Primary Cilia PCR Array	Human	Gene Expression	PAHS-127Z
Primary Cilia PCR Array	Mouse	Gene Expression	PAMM-127Z
Primary Cilia PCR Array	Rat	Gene Expression	PARN-127Z

Primary Cilia RT² Profiler™ PCR Array profiles the expression of 84 key genes important for ciliary organization and maintenance. The single primary cilium found on the surface of almost all mammalian cell types is a non-motile sensory organelle with a 9+0 microtubule formation. Mutations in key ciliary proteins have been recently linked to multiple diseases such as polycystic kidney disease (PKD) and Bardet-Biedl Syndrome (BBS), collectively called ciliopathies. The pathological mutations occur in genes necessary for cilia morphogenesis or maintenance via intraflagellar transport (IFT), disrupting primary cilia function. These discoveries provided the necessary biological significance to increase scientific interest and research in primary cilia. Recent studies suggest that primary cilia regulate the cell cycle, giving them a potential role in carcinogenesis. These organelles also coordinate with multiple signaling pathways such as Hedgehog, WNT and mTOR, although extensive research has not yet explained their connection with many disease phenotypes. This array contains genes important for signaling pathways central to ciliary function as well as genes important in cilia morphogenesis and maintenance. Using real-time PCR, you can easily and reliably analyze the expression of a focused panel of genes involved in primary cilia function with this array.
原纤毛PCR基因芯片可以同时检测与纤毛组织及其维护相关的84个关键基因的表达。原纤毛是在哺乳动物细胞的表面上发现的单一主要纤毛，是一个非能动的感觉细胞器，主要由9+0微管组成。关键的纤毛蛋白的突变与多种疾病相关，如多囊肾（PKD）和Bardet-Biedl综合征（BBS），合称为ciliopathies。发生在这些基因的病理突变会改变纤毛的形态或维持intraflagellar运输（IFT），从而扰乱原纤毛的功能。这些发现突出了对原纤毛科学研究的生物学意义。最近的研究表明，原纤毛参与细胞周期调节，并可能是一种潜在的致癌因素。这些细胞器也参与调控诸如Hedgehog，WNT和mTOR等信号转导通路。虽然仍没有具体的和许多疾病表型直接关联的研究，但这类信号转导通路的调控仍是研究热点。该芯片包含与纤毛形态发生和维护ZX信号通路相关的重要基因。利用实时定量PCR，研究者可以方便并且可信地对原纤毛功能相关基因进行同时检测。
Genes Mutated in Non-Motile Cilia Diseases:AHI1, ALMS1, ARL13B, ARL6, BBS1, BBS2, BBS4, BBS7,
CC2D2A, CEP290, GLIS2, HNF1B (TCF2), INVS, IQCB1, MKKS, MKS1, NEK8, NPHP1, NPHP3, PKD1, PKD2,PKHD1, RPGRIP1L, TMEM67 (MKS3), TTC8.
Intraflagellar Transport (IFT):DYNC2LI1, IFT172, IFT20, IFT74, IFT80, IFT88, KIF3A, KIF3B.
Cilium Morphogenesis:ALMS1, ARL6, BBS1, BBS2, BBS4, BBS7, IFT172, IFT88, MKKS, OFD1, PKHD1,
RPGRIP1L, VANGL2, WWTR1.
Cellular Signaling:
Hedgehog: BTRC (bTrCP), FUZ, GLI1, GLI2, GLI3, GSK3B, IHH, INTU, LRP2, PTCH1, RAB23, SHH, SMO, SUFU.
cAMP: ADCY3, ADCY7, AVPR2, HTR6, PKD2, SSTR3.
mTOR: AKT1, CDC42, GSK3B, IGF1, INS, MAPK1, MTOR, PIK3CA (p110a), PRKCA, RHOA, TP53, TSC1, TSC2.
Planar Cell Polarity (WNT): DVL1, FAT4, FJX1, FUZ, FZD1, INTU, RHOA, ROCK2, VANGL2, WNT9B.
WNT: AXIN2, INVS.
PDGFRa/Integrin: ITGB1, PDGFRA.
bRaf/MEK/ERK: FOS, KRAS, MAP2K1 (MEK1), MAPK1 (ERK2), MOS, PRKCA, PTPN5.
Cell Cycle:AKT1, BBS4, CCND1, CDK5RAP2, CDKN1A (p21CIP1/WAF1), IGF1, INS, MAP2K1, PKD1, PKD2,TP53.