肝miRNA发现者miRNA PCR芯片 Liver miFinder miScript miRNA PCR Array

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Liver miFinder miScript miRNA PCR Array

肝miRNA发现者miRNA PCR芯片
 

Product	Species	Technology	Cat. No.
Liver miFinder miScript miRNA PCR Array	Human	miRNA Expression	MIHS-116Z
Liver miFinder miScript miRNA PCR Array	Mouse	miRNA Expression	MIMM-116Z
Liver miFinder miScript miRNA PCR Array	Rat	miRNA Expression	MIRN-116Z

 
The Human Liver miFinder miScript miRNA PCR Array profiles the expression of 84 miRNAs abundantly expressed or best characterized in liver tissue. As the number of annotated miRNAs continues to expand, it becomes increasingly important to focus only on those miRNAs expressed in a particular cell line or tissue of interest, as not all miRNAs are expressed in every tissue. Due to this, we have experimentally analyzed the expression of miRNAs identified through miRBase V18 in a pool of human liver samples and correlated the results with scientific literature. Each of these miRNAs can regulate one or more messenger RNA transcripts, and conversely a given mRNA can be regulated by one or more miRNAs. Therefore, although they are well characterized, the complex role played by each miRNA is yet to be completely defined. This array maximizes the likelihood of discovering miRNAs whose expression patterns correlate with the biological phenotypes of liver tissue or hepatic cell lines under study. A set of controls present on each array enables data analysis using the ΔΔCT method of relative quantification, assessment of reverse transcription performance, and assessment of PCR performance. Using SYBR Green real-time PCR, the expression of a focused panel of miRNAs expressed in liver tissue can be easily and reliably analyzed with this miScript miRNA PCR Array.
miScript miRNA PCR Arrays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
肝miRNA发现者miRNA PCR芯片用于研究肝组织丰富表达或特异性表达的84 个miRNA。已注释的miRNA的数量不断扩大,ZD关注那些特异表达某种细胞系或组织的miRNA变得尤为重要,并非所有的miRNA在每一个组织中都表达。因此,我们通过实验在人类肝脏样本池通过miRBase V18分析已鉴定的miRNA的表达，并将结果与相关文献进行比较。每一种miRNA可以调节一个或多个信使RNA转录,反之一个给定的信使RNA可以由一个或多个miRNA调节。因此,尽管他们很有特点,每个miRNA的复杂作用尚未完全定义。这个芯片ZD化发现与肝组织或肝细胞系生物学表型相关的miRNA。每张芯片含一个对照组使得分析数据时可以用相对定量ΔΔCT方法评估逆转录效率,基因组DNA污染,和PCR效率。利用这张芯片，通过实时定量PCR，可以简易且可靠地肝中miRNA的表达。
 
miScript miRNA PCR芯片仅用于分子生物学应用。本产品不用于疾病的诊断、预防和ZL。
提供96孔板——384 -(4 x 96)板,和100孔板