人乳腺癌miRNAPCR芯片 Breast Cancer miScript miRNA PCR Array

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Breast Cancer miScript miRNA PCR Array

人乳腺癌miRNAPCR芯片
 

Product	Species	Technology	Cat. No.
Breast Cancer miScript miRNA PCR Array	Human	miRNA Expression	MIHS-109Z
Breast Cancer miScript miRNA PCR Array	Mouse	miRNA Expression	MIMM-109Z
Breast Cancer miScript miRNA PCR Array	Rat	miRNA Expression	MIRN-109Z

The Human Breast Cancer miScript miRNA PCR Array profiles the expression of 84 miRNAs known or predicted to alter their expression during breast cancer initiation or progression. This array provides cancer researchers with a convenient way to quickly analyze the miRNAs most relevant to breast tumorigenesis. Breast cancer is a heterogeneous disease with multiple classifications. For example, not all breast tumors express the estrogen receptor, necessary for treatment via selective estrogen receptor modulators (SERMs).In addition, some breast cancers progress under chemotherapeutic treatment and become resistant to certain drugs. Intense research into carcinogenic mechanisms has identified dysregulated genes, either via functional defects due to somatic mutations, or gene expression alterations due to epigenetic changes.miRNAs epigenetically regulate mRNA, and therefore miRNA dysregulation will affect target mRNA expression. Microarray expression analyses of miRNA have discovered potential biomarkers of breast cancer oncogenesis, and many results have been followed up with functional studies.This array includes miRNAs that are dysregulated in estrogen receptor-responsive or drug-resistant breast tumors. miRNAs with known functions in breast cancer are included, as well as miRNAs hypothesized to be involved, either via microarray studies or as a bioinformatically-predicted regulators of known breast cancer genes. The profiling results from this array may yield insights into the molecular mechanisms behind the pathogenesis of breast cancers. A set of controls present on this array enables data analysis using the ΔΔCT method of relative quantification, assessment of reverse transcription performance, and assessment of PCR performance. Using SYBR Green-based real-time PCR, the expression of a focused panel of miRNAs related to breast cancer can be easily and reliably analyzed with this miScript miRNA PCR Array.
miScript miRNA PCR Arrays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
 
人乳腺癌miRNAPCR芯片用于分析乳腺癌发生或发展过程中已知或预测发生表达变异的84 个miRNA的表达。这个芯片为癌症研究人员提供了一个方便的方法来快速分析乳腺肿瘤发生最相关的miRNA。乳腺癌是一种异质性疾病，包括多个种类。并不是所有的乳腺肿瘤都表达雌激素受体，需要通过选择性雌激素受体调节剂(SERMs)ZL。此外,一些乳腺癌的进程在化疗ZL中对某些药物产生耐药性。致癌机制的研究经确定了特异表达的基因，由于体细胞突变发生功能缺陷，或由于表观遗传变异基因表达改变。miRNA在表观遗传学上调节mRNA,因此miRNA的失调会影响靶mRNA的表达。miRNA表达芯片分析已被发现的乳腺癌肿瘤形成的潜在生物标记和跟进功能研究的许多结果中的miRNA。这个芯片包括雌激素受体-应答中特异表达或对乳腺肿瘤耐药的miRNA。包括功能已知以及通过miRNA芯片研究或已知乳腺癌生物调节剂推测涉及乳腺癌的miRNA。分析结果将助于了解乳腺癌病理背后的分子机制。每张芯片含一个对照组使得分析数据时可以用相对定量ΔΔCT方法评估逆转录效率,基因组DNA污染,和PCR效率。利用这张芯片，通过实时定量PCR，可以简易且可靠地分析乳腺癌关键miRNA的表达。
 
miScript miRNA PCR芯片仅用于分子生物学应用。本产品不用于疾病的诊断、预防和ZL。
Potential Biomarkers:
Upregulated:miR-128, miR-210.
Regulated:miR-7-5p.
Drug Resistance:
Upregulated:miR-10a-5p, miR-125b-5p, miR-132-3p, miR-155-5p, miR-186-5p, miR-199a-3p // miR-199b-3p, miR-21-5p, miR-210, miR-212-3p, miR-22-3p, miR-222-3p, miR-29a-3p, miR-31-5p, miR-328.
Downregulated:miR-141-3p, miR-148a-3p, miR-152, miR-15a-5p, miR-15b-5p, miR-181d, miR-193b-3p, miR-200c-3p, miR-205-5p, miR-25-3p, miR-27b-3p, miR-485-5p, miR-489, miR-93-5p.
Regulated:miR-100-5p, miR-107, miR-130a-3p, miR-140-5p, miR-17-5p // miR-106a-5p, miR-181b-5p, miR-181c-5p, miR-182-5p, miR-206, miR-495-3p.
Estrogen Receptor-Dependent:
Upregulated:miR-21-5p.
Downregulated:miR-181d, miR-193b-3p, miR-200a-3p, miR-200c-3p, miR-203a, miR-26a-5p, miR-26b-5p, miR-27a-3p, miR-27b-3p.
Regulated: let-7a-5p, let-7c, let-7f-5p, let-7g-5p, miR-181a-5p, miR-181b-5p, miR-98-5p.
miRNAs with Known Functions Related to Breast Cancer:let-7i-5p, miR-100-5p, miR-107, miR-10a-5p, miR-10b-5p, miR-125b-5p, miR-130a-3p, miR-132-3p, miR-140-5p, miR-141-3p, miR-148a-3p, miR-152, miR-155-5p, miR-15a-5p, miR-15b-5p, miR-16-5p, miR-17-5p // miR-106a-5p, miR-181d, miR-182-5p, miR-186-5p, miR-193b-3p, miR-199a-3p // miR-199b-3p, miR-200b-3p, miR-200c-3p, miR-204-5p, miR-205-5p, miR-206, miR-21-5p, miR-210, miR-212-3p, miR-22-3p, miR-222-3p, miR-25-3p, miR-27a-3p, miR-27b-3p, miR-29a-3p, miR-31-5p, miR-328, miR-429, miR-485-5p, miR-489, miR-495-3p, miR-7-5p, miR-93-5p, miR-96-5p.
miRNAs Predicted to Regulate Breast Cancer Genes:miR-1, miR-129-5p, miR-195-5p, miR-340-5p, miR-548c-3p, miR-607, miR-613.
Other miRNAs:let-7b-5p, let-7d-5p, let-7e-5p, miR-125b-1-3p, miR-130b-3p, miR-145-5p, miR-18a-5p, miR-19a-3p, miR-19b-3p, miR-199a-5p, miR-20a-5p, miR-20b-5p, miR-202-3p, miR-214-3p, miR-223-3p, miR-29b-3p, miR-29c-3p, miR-424-5p, miR-497-5p.

工作原理：

What miScript miRNA PCR Arrays offer?
   Guaranteed Performance* - ready-to-use for gene expression analysis
   Time & Cost Saving - less than 30 min hands-on time to analyze 84 or 372 miRNA
   Simple Data Analysis - easy-to-use Excel-Based Data Analysis Template