Product Description: IgG-Cutter
TM is a recombinant IgG endoproteinase (Mac/IdeS) from
Streptococcus pyogenes. It can specifically cleaves peptide bonds in the hinge region of IgG to form Fc and F(ab’)2 fragments. IgG-Cutter
TM cleaves all subclasses of human, rabbit, sheep, and monkey IgG with a high efficiency. In addition, IgG-Cutter
TM can cleave IgG2a and IgG3 of mouse IgG.
Product Source: IgG-Cutter
TM was produced in E. Coli cells transformed with Mac/IdeS gene. This product is sterile and does not contain any components of
animal origin. | Catalog # | Size | Concentration | price |
| ###### | 2,500 units | 50 units/ul | free for test |
Reaction Conditions: 20 mM Tris buffer pH 8.0; incubate at 37°C for at least 30 minutes. Cleavage reaction can also be carried out at room temperature using longer time.
Unit Definition: One unit is defined as the amount of enzyme required to digest >95% of 1 ug of human IgG in 30 minutes at 37°C.
Form: Sterile liquid formulation. 20 mM Tris buffer pH 8.0, 50% Glycerol, and 5 mM b-Mercaptoethanol.
Usage: FOR LABORATORY RESEARCH USE ONLY.
Quality Control Assays Purity: Greater than 95% as determined by SDS-PAGE.
Activity: Each lot of IdeS has been tested by the IgG cleavage assay.
New
1 2 3 4 5 
Figure 1. IgG cleavage by IgG-CutterTM. Lanes: 1, IgG-Cutter
TM with a mass of 34 kDa on SDS-PAGE; lane 2, protein marker; lane 3, protein marker; lane 4, 10 ug of IgG antibody before IgG-Cutter
TM treatment; lane 5,10 ug of IgG antibody after IgG-Cutter
TM treatment (10 units for 30 minutes at 37°C). All samples were reduced with b-Mercaptoethanol before SDS-PAGE.
Phophate buffer saline(PBS) pH 6.0-8.0
Tris buffer pH 7.0-8.0
MES buffer pH 5.5-6.5
HEPES buffer 7.0-8.0
Ammonium bicarbonate buffer 6.0-7.0
Sodium acetate buffer 6.0
Background: IgG-Cutter
TM is recombinant IgG endoproteinase (Mac/IdeS) from S
treptococcus pyogenes. The complete genome sequencing of M1 group A
Streptococcus (GAS) was done in 2001 (Ferretti 2001). To our knowledge, Mac/IdeS was first identified in group A
Streptococcus (GAS) infected mice and human sera as a homologous protein to Human Mac-1 at NIH (Lei 2000). Mac/IdeS was later found to promote inhibition of opsonophagocytosis, enhancement of pathogen survival, and establishment of infection and dissemination (Lei 2001). Several groups later characterized Mac/IdeS’s IgG endoproteinase activity, and found it to cleave peptide bonds in the hinge region of IgG (von Pawel-Rammingen 2002, Lei 2002).
References: - Ferretti JJ, McShan WM, Ajdic D. et al (2001) Proc.Natl. Acad. Sci. USA, 98, 4658-4663.
- Lei, B., Mackie, S., Lukomski, S., Musser, M. M. (2000) infect. Immun. 68, 6807-6818.
- Lei B, Deleo FR, Hoe NP. Et al (2001) Nat. Med. 7, 1298-1305
- 2. von Pawel-Rammingen, U., Johansson, B. P. & Bjorck, L. (2002) EMBO J. 21, 1607-1615.
- Lei, B., DeLeo, F. R., Reid, S. D., Voyich, J. M., Magoun, L., Liu, M., Braughton, K. R., Ricklefs, S., Hoe, N. P., Cole, R. L., et al. (2002) infect. Immun. 70, 6880-6890.
温馨提示：不可用于临床ZL。
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