核仁素(NCL)多克隆抗体 Polyclonal Antibody to Nucleolin (NCL)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | XYC242Hu01 |
| Clonality | Polyclonal |
| Host | Rabbit |
| Immunoglobulin Type | IgG |
| Purification | Affinity Chromatography |
| Applications | WB, ICC, IHC-P, IHC-F, ELISA |
| Concentration | 200ug/ml |
| UOM | 100ug |
| Conjugate | No Conjugate |
核仁素(NCL)多克隆抗体 ANTIBODY SPECIFITY
The antibody is a rabbit polyclonal antibody raised against NCL. It has been selected for its ability to recognize NCL in immunohistochemical staining and western blotting.
APPLICATIONS
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Optimal working dilutions must be determined by end user.
CONTENTS
Form & Buffer: Supplied as solution form in PBS, pH7.4,
containing 0.02% NaN3, 50% glycerol.
核仁素(NCL)多克隆抗体 QUALITY CONTROL
Content: The quality control contains recombinant NCL (Lys388~Gln569) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
核仁素(NCL)多克隆抗体 STORAGE
Store at 4oC for frequent use. Stored at -20oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
