聚集蛋白聚糖(AGC)重组蛋白 Recombinant Aggrecan (AGC)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Bos taurus; Bovine (Cattle) |
| Product No. | |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 32.4kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Residues | Glu2066~Arg2316 (Accession # P13608) with two N-terminal Tags, His-tag and T7-tag |
聚集蛋白聚糖(AGC)重组蛋白 | Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
The sequence of the target protein is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- EARLE IESSSPVHSG EESQTADTAT SPTDASIPAS AGGTDDSEAT TTDQKLCEEG WTKFQGHCYR HFPDRATWVD AESQCRKQQS HLSSIVTPEE QEFVNNNAQD YQWIGLNDKT IEGDFRWSDG HSLQFENWRP NQPDNFFATG EDCVVMIWHE KGEWNDVPCN YQLPFTCKKG TVACGEPPVV EHARIFGQKK DRYEINALVR YQCTEGFIQG HVPTIRCQPS GHWEEPRITC TDPATYKRRL QKRSSR
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
聚集蛋白聚糖(AGC)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
聚集蛋白聚糖(AGC)重组蛋白Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
温馨提示：不可用于临床ZL。
