Horseradish Peroxidase Native cardiac Troponin complex antibody conjugate (Tcom8)

The troponin complex consists of regulatory proteins troponin C (TnC), troponin I (TnI) and troponin T (TnT). These proteins are bound to the thin filaments of muscle fibres and are involved in the regulation of calcium dependent striated and cardiac muscle contraction. During muscle excitation, calcium ions bind to TnC resulting in a conformational change that exposes a site to which troponin I binds. Troponin I inhibits the interaction of myosin with actin and the TnT subunit stabilises the TnC/TnI complex by fixing it to the actin-tropomyosin filament.The troponin subunits are all encoded by separate genes. Two forms of TnC exist, one of which is expressed in skeletal muscles and the other in myocardium. Different tissue specific forms of TnI and TnT also exist with distinct distribution in slow skeletal muscle, fast-twitch skeletal muscle and cardiac muscle. Cardiac TnI (cTnI) and TnT (cTnT) is primarily bound to myofibrils in the cardiac myocytes but a small percentage exists in a cytosolic pool. Following continued myocardial injury, concentrations of both cTnI and cTnT become elevated in the bloodstream. Studies show that cTnI may circulate in the bloodstream in various forms either as free TnI, shortly after injury, or as a complex with TnC or with cTnT/TnC. In the clinical setting, cTnI and cTnT are considered to be sensitive and specific biomarkers for myocardial injury and are widely used in the assessment of cardiac muscle death, and the diagnosis of myocardial infarction.