PerCP anti-mouse/human CD11b_详细资料

产品信息

抗体名： CD11b 抗体抗体英文名： CD11b antibody 靶点：克隆M1/70 浓度： 0.2mg/ml 应用范围： FC - Quality tested 宿主： Rat 适应物种： Mouse, Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Rabbit (Lapine) 标记物： FC - Quality tested 克隆性：多克隆亚型： IgG2b, κ 免疫原： C57BL/10 splenocytes 规格： 25 ug

Clone M1/70

Isotype Rat IgG2b, κ

Reactivity Mouse, Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Rabbit (Lapine)

Immunogen C57BL/10 splenocytes

Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions. The solution is free of unconjugated PerCP and unconjugated antibody.

Concentration 0.2mg/ml

Recommended Usage

Applications
FC - Quality tested

Application Notes
Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1,4, in vitroblocking3,9,12, depletion2,8, immunofluorescence microscopy6,7,10, and immunohistochemistry of acetone-fixed frozen sections5,11-13 and paraffin sections28. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 101231). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 101248) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References
1. Springer T, et al. 1978. Eur. J. Immunol. 8:539. (IP)
2. Ault K and Springer T. 1981. J. Immunol. 126:359. (Deplete)
3. Springer TA, et al. 1982. Immunol. Rev. 68:171. (Block)
4. Ho MK and Springer TA. 1983. J. Biol. Chem. 258:2766. (IP)
5. Flotte TJ, et al. 1983. Am. J. Pathol. 111:112. (IHC)
6. Noel GJ, et al. 1990. J. Clin. Invest. 85:208. (IF)
7. Allen LA and Aderem A. 1996. J. Exp. Med. 184:627 (IF)
8. D'Amico A and Wu L. 2003. J. Exp. Med. 198:293. (Deplete)
9. Brickson SJ, et al. 2003. Appl Physiol. 95:969. (Block)
10. Clatworthy MR and Smith KG. 2004. J. Exp. Med. 199:717. (IF)
11. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
12. Zhang Y, et al. 2002. J. Immunol. 168:3088. (IHC)
13. Iwasaki A and Kelsall BL. 2001. J. Immunol. 166:4884 (IHC, FC)
14. Tailleux L. 2003. J. Exp. Med. 197:121. (Block, FC)
15. Olver S, et al. 2006. Cancer Research 66:571. (FC)
16. Tan SL, et al. 2006. J. Immunol. 176:2872. (FC) PubMed
17. Ponomarev ED, et al. 2006. J. Immunol. 176:1402. (FC)
18. Dzhagalov I, et al. 2007. Blood 109:1620. (FC)
19. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
20. Rasmussen JW, et al. 2006. Infect. Immun.74:6590. PubMed
21. Napimoga MH, et al. 2008. J. Immunol. 180:609. PubMed
22. Elqaraz-Carmon V, et al. 2008. J. Lipid. Res. 49:1894. PubMed
23. Kim DD, et al. 2008. Blood 112:1109. PubMed
24. Guo Y, et al. 2008. Blood 112:480. PubMed
25. Norian LA, et al. 2009. Cancer Res. 69:3086. (FC) PubMed
26. Baumgartner CK, et al. 2010. J. Immunol. 184:573. PubMed
27. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
28. Whiteland J, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
"

Description

Storage The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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Clone	M1/70
Isotype	Rat IgG2b, κ
Reactivity	Mouse, Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Rabbit (Lapine)
Immunogen	C57BL/10 splenocytes
Formulation	Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation	The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions. The solution is free of unconjugated PerCP and unconjugated antibody.
Concentration	0.2mg/ml
Recommended Usage
Applications	FC - Quality tested
Application Notes	Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1,4, in vitroblocking3,9,12, depletion2,8, immunofluorescence microscopy6,7,10, and immunohistochemistry of acetone-fixed frozen sections5,11-13 and paraffin sections28. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 101231). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 101248) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application References	1. Springer T, et al. 1978. Eur. J. Immunol. 8:539. (IP) 2. Ault K and Springer T. 1981. J. Immunol. 126:359. (Deplete) 3. Springer TA, et al. 1982. Immunol. Rev. 68:171. (Block) 4. Ho MK and Springer TA. 1983. J. Biol. Chem. 258:2766. (IP) 5. Flotte TJ, et al. 1983. Am. J. Pathol. 111:112. (IHC) 6. Noel GJ, et al. 1990. J. Clin. Invest. 85:208. (IF) 7. Allen LA and Aderem A. 1996. J. Exp. Med. 184:627 (IF) 8. D'Amico A and Wu L. 2003. J. Exp. Med. 198:293. (Deplete) 9. Brickson SJ, et al. 2003. Appl Physiol. 95:969. (Block) 10. Clatworthy MR and Smith KG. 2004. J. Exp. Med. 199:717. (IF) 11. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC) 12. Zhang Y, et al. 2002. J. Immunol. 168:3088. (IHC) 13. Iwasaki A and Kelsall BL. 2001. J. Immunol. 166:4884 (IHC, FC) 14. Tailleux L. 2003. J. Exp. Med. 197:121. (Block, FC) 15. Olver S, et al. 2006. Cancer Research 66:571. (FC) 16. Tan SL, et al. 2006. J. Immunol. 176:2872. (FC) PubMed 17. Ponomarev ED, et al. 2006. J. Immunol. 176:1402. (FC) 18. Dzhagalov I, et al. 2007. Blood 109:1620. (FC) 19. Fazilleau N, et al. 2007. Nature Immunol. 8:753. 20. Rasmussen JW, et al. 2006. Infect. Immun.74:6590. PubMed 21. Napimoga MH, et al. 2008. J. Immunol. 180:609. PubMed 22. Elqaraz-Carmon V, et al. 2008. J. Lipid. Res. 49:1894. PubMed 23. Kim DD, et al. 2008. Blood 112:1109. PubMed 24. Guo Y, et al. 2008. Blood 112:480. PubMed 25. Norian LA, et al. 2009. Cancer Res. 69:3086. (FC) PubMed 26. Baumgartner CK, et al. 2010. J. Immunol. 184:573. PubMed 27. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed 28. Whiteland J, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC) "
Description
Storage	The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.