Human TNF-α ELISA MAX™ Deluxe

ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price! BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use. Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.   Kit Contents: Human TNF-α ELISA MAX™ Capture Antibody (200X) Human TNF-α ELISA MAX™ Detection Antibody (200X) Human TNF-α Standard Avidin-HRP (1000X) Substrate Solution A Substrate Solution B Coating Buffer A (5X) Assay Diluent A (5X) NUNC Maxisorp™ 96 MicroWell Plates   Materials Not Included: A microplate reader capable of measuring absorbance at 450 nm Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL Deionized (DI) water PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered. Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH=7.4). BioLegend Cat. No. 421601 is recommended. Wash bottle or automated microplate washer Stop Solution (e.g. 2N H2SO4) Biolegend Cat. No. 423001 is recommended Log-Log graph paper or software for data analysis Tubes to prepare standard dilutions Timer Plate Sealer Absorbent paper