Rat Alpha 2 Heremans Schmid Glycoprotein(AHSG) ELISA Kit

Rat Alpha 2 Heremans Schmid Glycoprotein(AHSG) ELISA Kit标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml    OD1    OD2    Average        
8000    0.078    0.075    0.077        
4000    0.145    0.156    0.151        
2000    0.233    0.245    0.239        
1000    0.396    0.375    0.386        
500    0.645    0.655    0.650        
250    0.943    0.932    0.938        
125    1.107    1.112    1.110        
0    1.612    1.603    1.608        Rat Alpha 2 Heremans Schmid Glycoprotein(AHSG) ELISA Kit精密度:
Intra-assay Precision (Precision within an assay): CV%<8%            
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%            
Three samples of known concentration were tested in twenty assays to assess.        Rat Alpha 2 Heremans Schmid Glycoprotein(AHSG) ELISA Kit线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat AHSG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample    Serum(n=4)    
1:100    Average %    99    
Range %    95-103    
1:200    Average %    103    
Range %    98-108    
1:400    Average %    89    
Range %    84-94    
1:800    Average %    96    
Range %    92-100    Rat Alpha 2 Heremans Schmid Glycoprotein(AHSG) ELISA Kit回收率:
The recovery of rat AHSG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type    Average % Recovery    Range    
Serum (n=5)    93    89-99    
EDTA plasma (n=4)    104    102-107