胸素β10抗原 0.5ml T Beta10 peptide
DISC1 N端抗原 0.5mg DISC1(disrupted in schizophrenia1)(NT)
神经激肽B受体(抗原) 0.5mg NKR(Neuromedin B Receptor)
人锌指脂蛋白-1c(抗原) 0.5mg ZNF268 (zinc finger protein ZNF268)- middle (1c)
上皮细胞粘附分子/表皮细胞粘附分子 0.5mg EpCAM
Nogo-66 (1-40) 0.5mg Nogo-66 1-40(NEP 1-40)
荧光素标记马IgM 0.3ml Horse IgM/FITC
荧光素PE标记鸡IgG 0.1ml Chicken IgG/PE
荧光素Cy3标记猪IgG 0.1ml pig IgG/Cy3
红色荧光素罗丹明标记链霉亲和素 0.3ml Streptavidin/RBITC
人B-淋巴细胞趋化因子 96T BLC-1/CXCL13(Human B-Lymphocyte Chemoattractant 1,BLC-1) ELISA Kit
巨细胞病毒 IgG(间接法二步法) CMV
流感病毒A IgG(间接法二步法) ELISA试剂盒 48T IFV IgG/IFV-A IgG
大鼠Ⅰ型胶原C端肽 96T (CTX- I )ELISA Kit
小鼠嗜酸性粒细胞阳离子蛋白 96T Mouse eosinophil catio
nic protein,ECP ELISA Kit
人可溶性细胞因子受体 96T sCKR(Human soluble cytokine receptor) ELISA Kit
大鼠钙调磷酸酶 96T CaN ELISA Kit
人血管内皮细胞生长因子受体1 96T VEGFR-1/Flt1(Human Vascuoar endothelial cell growth factor receptor 1) ELISA kit PLC thio-PIP2 -sodium salt) (5 mg) PLC thio-PIP2 -sodium salt), 3-(3-(1,2-dihydroxy-1,2-dihexanoyl)thiopropane)inositol-4,5-diphosphate, trisodium salt
8-iso Misoprostol (500 ug) 8-iso Misoprostol, 9-oxo-11.alpha.,16-dihydroxy-16-methyl-(8.beta.)-prost-13E-en-1-oic acid, methyl ester
G-418 Sulfate (500 mg) Geneticin, G-418 Sulfate, O-2-amino-2,7-dideoxy-D-glycero-α-D-gluco-heptopyranosyl-(1→4)-O-[3-deoxy-4-C-methyl-3-(methylamino)-β-L-arabinopyranosyl-(1→6)]-2-deoxy-sulfate-D-streptamine
Petromyzo
nol AChE Tracer (100 dtn) Petromyzo
nol AChE Tracer
(R)-(-)-Linoleyl-2 -Hydroxy-1 -Propylamide (10 mg) (R)-(-)-Linoleyl-2 -Hydroxy-1 -Propylamide, N-((R)-2-hydroxypropyl)octadeca-9Z,12Z-dienamide
硝铵(500GM) Ammo
nium Nitrate, CAS# 6484-52-2; ≥98%; HAZARDOUS MATERIAL
L-谷胱甘肽(1KG) L-Glutamine, CAS# 56-85-9; MW 146.14; C5H10N2O3; USP Grade; Endotoxin and cell culture tested.
无水
表达小鼠MHCⅠ类分子Kb的人胚肾细胞;293KB价格磷氢二钠(100GM) Sodium Phosphate, Dibasic, Anhydrous, ACS Grade; Cas# 7558-79-4; Formula: Na2HPO4; Formula Weight: 141.96
青链霉素溶液(100X)(6X100ML) Penicillin-Streptomycin Solution (100X), Co
ntains 10,000 U/ml of penicillin and 10,000 ug/ml streptomycin in a 0.9% saline sterile-filtered solution.
5(6)-ROX[5(6)-羧基-X-罗丹明,琥珀酰亚胺酯] 混合物 5(6)-ROX, SE [5-(and-6)-Carboxy-X-rhodamine, succinimidyl ester] Mixed isomers
藻红蛋白 R-PE (R-Phycoerythrin)
20S蛋白酶体荧光底物(Ac-ANW)2R110 (Ac-ANW)2R110
表达小鼠MHCⅠ类分子Kb的人胚肾细胞;293KB价格操作步骤:
1)贴壁细胞传代:提前将培养基、PBS放入37℃水浴锅内预热,用75%酒精擦拭后再放入超净台内,吸除或倒掉细胞瓶内旧培养液,加少量PBS润洗细胞,加入适量胰酶,使胰酶的量能盖住细胞,37℃孵育,每隔2~3min显微镜下观察,待贴壁细胞间间隙变大、细胞趋于圆形但还未漂起时弃去胰酶,加入新鲜培养基,晃动细胞瓶,终止胰酶作用,用吸管小心吹打贴壁的细胞,制成细胞悬液。控制吹打的力度,避免产生大量的气泡,将细胞悬液分别接种到另外的2~3个细胞瓶内,加入新鲜培养基,置37℃温箱培养,隔天观察贴壁生长情况。
2)悬浮细胞传代:将细胞悬液转移到无菌离心管内,1000rpm离心5min,弃去上清,加入新鲜的培养基,用吸管小心吹散沉淀,制成细胞悬液,将细胞悬液分别接种到另外的2~3个细胞瓶内,加入新鲜培养基,置37℃温箱培养。