Rat Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial(AADAT) ELISA kit

Rat Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial(AADAT) ELISA kitPRINCIPLE OF THE ASSAY 
This assay employs the quantitative sandwich enzyme immunoassay technique. 
Antibody specific for AADAT has been pre-coated onto a microplate. Standards 
and samples are pipetted into the wells and any AADAT present is bound by the 
immobilized antibody. After removing any unbound substances, a 
biotin-conjugated antibody specific for AADAT is added to the wells. After 
washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. 
Following a wash to remove any unbound avidin-enzyme reagent, a substrate 
solution is added to the wells and color develops in proportion to the amount of 
AADAT bound in the initial step. The color development is stopped and the 
intensity of the color is measured. Rat Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial(AADAT) ELISA kitDETECTION RANGE 
31.2 pg/ml-2000 pg/ml. Rat Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial(AADAT) ELISA kitSENSITIVITY 
The minimum detectable dose of rat AADAT is typically less than 7.8 pg/ml. 
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as 
the lowest protein concentration that could be differentiated from zero. It was 
determined the mean O.D value of 20 replicates of the zero standard added by 
their three standard deviations. 
SPECIFICITY 
This assay has high sensitivity and excellent specificity for detection of rat 
AADAT. No significant cross-reactivity or interference between rat AADAT and 
analogues was observed. 
Note: Limited by current skills and knowledge, it is impossible for us to complete 
the cross-reactivity detection between rat AADAT and all the analogues, 
therefore, cross reaction may still exist.  
3 Rat Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial(AADAT) ELISA kitPRECISION 
Intra-assay Precision (Precision within an assay): CV%<8% 
Three samples of known concentration were tested twenty times on one plate to 
assess. 
Inter-assay Precision (Precision between assays): CV%<10% 
Three samples of known concentration were tested in twenty assays to assess. 
LIMITATIONS OF THE PROCEDURE 
 FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC 
PROCEDURES. 
 The kit should not be used beyond the expiration date on the kit label. 
 Do not mix or substitute reagents with those from other lots or sources. 
 If samples generate values higher than the highest standard, dilute the 
samples with Sample Diluent and repeat the assay. 
 Any variation in Sample Diluent, operator, pipetting technique, washing 
technique, incubation time or temperature, and kit age can cause variation 
in binding. 
 This assay is designed to eliminate interference by soluble receptors, 
binding proteins, and other factors present in biological samples. Until all 
factors have been tested in the Immunoassay, the possibility of 
interference cannot be excluded