细胞名称 人肾细胞腺癌细胞;ACHN保存
形态特性 上皮细胞
生长特性 贴壁生长
特征特性 人干扰素可YZ其生长[Hogan, T. F., 个人通讯]。 ACHN或能用于人干扰素、干扰素诱导因子的抗肿瘤活性研究。
培养条件 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle's Balanced Salts) 优质胎牛血清,10%
传代方法 消化3-5分钟。1:2。3天内可长满。
传代情况 PN5
冻存条件 完全培养基+8%DMSO
支原体检测 阴性
STR
同工酶
染色体
使用权限 A类
我们的细胞株来源于ATCC、ECACC、DSMZ、JCRB等,购买人肾细胞腺癌细胞;ACHN保存到货后,由我们实验室扩增、冻存,冻存的产品全部经过QC检测,进口来源,保证5代以内,活力>95%,无细菌、真菌、支原体污染。
人肾细胞腺癌细胞;ACHN保存操作步骤:
1)贴壁细胞传代:提前将培养基、PBS放入37℃水浴锅内预热,用75%酒精擦拭后再放入超净台内,吸除或倒掉细胞瓶内旧培养液,加少量PBS润洗细胞,加入适量胰酶,使胰酶的量能盖住细胞,37℃孵育,每隔2~3min显微镜下观察,待贴壁细胞间间隙变大、细胞趋于圆形但还未漂起时弃去胰酶,加入新鲜培养基,晃动细胞瓶,终止胰酶作用,用吸管小心吹打贴壁的细胞,制成细胞悬液。控制吹打的力度,避免产生大量的气泡,将细胞悬液分别接种到另外的2~3个细胞瓶内,加入新鲜培养基,置37℃温箱培养,隔天观察贴壁生长情况。
2)悬浮细胞传代:将细胞悬液转移到无菌离心管内,1000rpm离心5min,弃去上清,加入新鲜的培养基,用吸管小心吹散沉淀,制成细胞悬液,将细胞悬液分别接种到另外的2~3个细胞瓶内,加入新鲜培养基,置37℃温箱培养。
人肾细胞腺癌细胞;ACHN保存的特点:
1) 特异性强:只对肿瘤细胞进行有效识别和杀灭,而不伤害正常组织和细胞;抗瘤谱广。
2) 对体内各种肿瘤细胞均能有效ZL。
3) 安全性好,除可能出现的发热、少量出血外,无其他不良反应。
分裂图展示图:
人肾细胞腺癌细胞;ACHN保存操作台的灭菌处理:
1)无菌室及无菌操作台(laminarflow)用紫外灯照射30-60分钟灭菌,70%ethanol擦拭无菌操作抬面,并开启无菌操作台风扇运转10分钟。2)细胞用的培养基如有相同切记不可共享培养基,以避免失误混淆或细胞间污染。 (注:实验操作应在抬面之ZY无菌区域,勿在边缘之非无菌区域操作。)培养细胞将复合细胞计数要求的细胞悬液分装入培养瓶内,将培养瓶放入37℃和5%CO2的培养箱内2-4小时(或者24-48小时)后换液继续培养培养,换液的时间由细胞情况而定。
ELISA Kit for Human Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial小鼠骨形成蛋白8B(BMP8B)ELISA试剂盒,英文名:BMP8B ELISA Kit
人缺氧诱导基因2蛋白(HIG2)ELISA试剂盒人96T0.625-40 ng/mL小鼠钙网蛋白(CRT)ELISA试剂盒,英文名:CRT ELISA Kit
ELISA Kit for Human High-density lipoprotein小鼠毒蕈碱型胆碱受体M2(CHRM2)ELISA试剂盒,英文名:CHRM2 ELISA Kit
人整合蛋白beta-3 ELISA试剂盒人96T0.078-5 ng/ml小鼠成纤维细胞生长因子受体1(FGFR1)ELISA试剂盒,英文名:FGFR1 ELISA Kit
ELISA Kit for Human Interferon alpha-2小鼠白细胞衍生趋化因子1(LECT1)ELISA试剂盒,英文名:LECT1 ELISA Kit
人白介素26(IL-26)ELISA试剂盒人96T15.6-1000 pg/mL小鼠氨基端前脑钠素(NT-ProBNP)ELISA试剂盒,英文名:NT-ProBNP ELISA Kit
ELISA Kit for Human Insulin receptor小鼠Slit同源物2(Slit2)ELISA试剂盒,英文名:Slit2 ELISA Kit
人白介素5受体αELISA试剂盒人96T78-5000 pg/mL小鼠EGF样域蛋白7(EGFL7)ELISA试剂盒,英文名:EGFL7 ELISA Kit
ELISA Kit for Human Insulin-like growth factor-binding protein 1小鼠Ⅳ型胶原(COL4)ELISA试剂盒,英文名:COL4 ELISA Kit
人kelch样蛋白17(klhl17)ELISA试剂盒人96T15.6-1000 pg/mL大鼠总胆红素(TB)ELISA试剂盒,英文名:TB ELISA Kit
ELISA Kit for Human La-related protein 7大鼠脂多糖/内毒素(LPS)ELISA试剂盒,英文名:LPS ELISA Kit
人乳糖凝集素1(LGALS1)ELISA试剂盒人96T0.78-50 ng/mL大鼠乙醛脱氢酶(ALDH)ELISA试剂盒,英文名:ALDH ELISA Kit
ELISA Kit for Human Neural cell adhesion molecule L1大鼠血小板膜糖蛋白ⅡbⅢa(GP-ⅡbⅢa/CD41+CD61)ELISA试剂盒,英文名:GP-ⅡbⅢa/CD41+CD61 ELISA Kit
甲基丙二酸(Methylmalonic Acid)ELISA试剂盒通用96T0.312-20 ng/mL大鼠胸腺基质淋巴细胞生成素(TSLP)ELISA试剂盒,英文名:TSLP ELISA Kit
ELISA Kit for Human Mesenteric estrogen-dependent adipogenesis protein大鼠胃泌素(Gastrin)ELISA试剂盒,英文名:Gastrin ELISA Kit
人基质金属蛋白酶14(MMP-14)ELISA试剂盒人96T31.2-2000 pg/mL大鼠碳酸酐酶2(CA-2)ELISA试剂盒,英文名:CA-2 ELISA Kit
ELISA Kit for Human Mitochondrial peptide methionine sulfoxide reductase大鼠神经特异性烯醇化酶(NSE)ELISA试剂盒,英文名:NSE ELISA Kit
人粘蛋白2(MUC2)ELISA试剂盒人96T0.156-10 ng/mL大鼠前列腺素F2α(PGF2α)ELISA试剂盒,英文名:PGF2α ELISA Kit
ELISA Kit for Human Collagenase 3大鼠内皮素转换酶1(ECE1)ELISA试剂盒,英文名:ECE1 ELISA Kit
人NADPH氧化酶1(NOX-1)ELISA试剂盒人96T0.78-50 ng/mL大鼠磷蛋白关联鞘糖脂微区1(PAG1)ELISA试剂盒,英文名:PAG1 ELISA Kit
ELISA Kit for Human Neuromedin-U大鼠抗内皮细胞抗体(AECA)ELISA试剂盒,英文名:AECA ELISA Kit
人神经突蛋白1(NRN-1)ELISA试剂盒人96T0.156-10 nmol/L大鼠碱性成纤维生长因子(bFGF)ELISA试剂盒,英文名:bFGF ELISA KitGinsenoside Rb1 (5 mg) Gypenoside III|NSC 310103; 20-[(6-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]-12β-hydroxydammar-24-en-3β-yl 2-O-β-D-glucopyranosyl-β-D-glucopyranoside
Heat Shock Protein Inhibitor I (10 mg) HSP Inhibitor I|KNK 437; 3-(1,3-benzodioxol-5-ylmethylene)-2-oxo-1-pyrrolidinecarboxaldehyde
11β-Prostaglandin F1β (500 ug) 11-epi PGF1β|11β-PGF1β|9β,11β-PGF1α; 9β,11β,15S-trihydroxy-prost-13E-en-1-oic acid
UCF 101 (5 mg) dihydro-5-[[5-(2-nitrophenyl)-2-furanyl]methylene]-1,3-diphenyl-2-thioxo-4,6(1H,5H)-pyrimidinedione
JNK Inhibitor IX (50 mg) TCS JNK 5a|c-Jun N-terminal Kinase Inhibitor IX; N-(3-cyano-4,5,6,7-tetrahydrobenzo[b]thien-2-yl)-1-naphthalenecarboxamide
Fialuridine (1 mg) 5-Iodo-2’-Fluoroarauracil|FIAU|Fluoroiodoarauracil|NSC 678514; 1-(2-deoxy-2-fluoro-β-D-arabinofuranosyl)-5-iodo-2,4(1H,3H)-pyrimidinedione
OTX015 (5 mg) HY-15743; (6S)-4-(4-chlorophenyl)-N-(4-hydroxyphenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepine-6-acetamide
Vitamin K1 2,3-epoxide (500 ug) 2,3-Epoxyphylloquinone|Phylloquinone oxide|Vitamin K oxide; 1a,7a-dihydro-1a-methyl-7a-(3,7,11,15-tetramethyl-2-hexadecen-1-yl)-naphth[2,3-b]oxirene-2,7-dione
N- NANA|Neuc; N-acetyl-neuraminic acid
20-Hydroxyecdysone (5 mg) Ecdysterone|Isoinokosterone; (5β)-2β,3β,14,20,22R,25-hexahydroxy-cholest-7-en-6-one
Chlorthalidone (10 mg) Hygroton|NSC 69200|Oradil|Thalitone; 2-chloro-5-(2,3-dihydro-1-hydroxy-3-oxo-1H-isoindol-1-yl)-benzenesulfonamide
ONX 0912 (5 mg) Oprozomib|PR 047; O-
SU 1498 (1 mg) AG-1498|Tyrphostin SU 1498; (2E)-2-cyano-3-[4-hydroxy-3,5-bis(1-methylethyl)phenyl]-N-(3-phenylpropyl)-2-propenamide
人肾细胞腺癌细胞;ACHN保存Nullscript (10 mg) N-hydroxy-1,3-dioxo-1H-benz[de]isoquinoline-2(3H)-butanamide
Gimeracil 5-Chlorodihydropyrimidine|CDHP|Gimestat; 5-chloro-4-hydroxy-2(1H)-pyridinone
Baricitinib (10 mg) INCB 028050|LY3009104; 1-(ethylsulfonyl)-3-[4-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)-1H-pyrazol-1-yl]-3-azetidineacetonitrile
Ximelagatran Exanta|H 376/95; N-[(1R)-1-cyclohexyl-2-[(2S)-2-[[[[4-[(hydroxyamino)iminomethyl]phenyl]methyl]amino]carbonyl]-1-azetidinyl]-2-oxoethyl]-glycine, ethyl ester
AB-CHMINACA metabolite M7 (5 mg) 2-(1-(cyclohexylmethyl)-1H-indazole-3-carboxamido)-3-methylsuccinic acid
2-Bromoamphetamine (hydrochloride) (10 mg) 2-BA|o-BA; 2-bromo-α-methyl-benzeneethanamine, monohydrochloride
Seratrodast (10 mg) A-73001|AA2414|ABT-001; ζ-
AT-7519 (hydrochloride) (25 mg) 4-[(2,6-dichlorobenzoyl)amino]-N-4-piperidinyl-1H-Pyrazole