Recombinant HIV-2 gp32, Horseradish Peroxidase Labled

Introduction  HIV-1 and HIV-2 appear to package their RNA differently. HIV-1 binds to any appropriate RNA whereas HIV-2 preferentially binds to mRNA which creates the Gag protein itself. This means that HIV-1 is better able to mutate. HIV-2 is transmitted in the same ways as HIV-1: Through exposure to bodily fluids such as blood, semen, tears and vaginal fluids.

Immunodeficiency develops more slowly with HIV-2.

HIV-2 is less infectious in the early stages of the virus than with HIV-1.

The infectiousness of HIV-2 increases as the virus progresses.

Major differences include reduced pathogenicity of HIV-2 relative to HIV-1, enhanced immune control of HIV-2 infection and often some degree of CD4-independence. Despite considerable sequence and phenotypic differences between HIV-1 and 2 envelopes, structurally they are quite similar. Both membrane-anchored proteins eventually form the 6-helix bundles from the N-terminal and C-terminal regions of the ectodomain, which is common to many viral and cellular fusion proteins and which seems to drive fusion.

HIV-1 gp41 helical regions can form more stable 6-helix bundles than

HIV-2 gp41 helical regions however HIV-2 fusion occurs at a lower threshold temperature (25°C), does not require Ca2+ in the medium, is insensitive to treatment of target cells with cytochalasin B, and is not affected by target membrane glycosphingolipid composition. 

Recombinant HIV-2 gp32, Horseradish Peroxidase LabledDescription  HIV-2 gp32 HRP Labeled recombinant- contains the full-length sequence of HIV-2 envelope immunodominant regions gp32. 

Source  Escherichia Coli. 

Physical Appearance  Sterile filtered colorless clear solution. 

Formulation  20mM PBS pH-7.8, NaCl 0.5M, DTT 1mM, 8M urea & 0.4M imidasole. 

Recombinant HIV-2 gp32, Horseradish Peroxidase LabledPurity Greater than 95.0% as determined by HPLC analysis and SDS-PAGE. 

Stability HIV-2 gp32, HRP although stable at 4°C for 1 week, should be stored below -18°C.

Please prevent freeze thaw cycles. 

Specificity  Immunoreactive with all sera of HIV-2 infected individuals. 

Applications  HIV-2 gp32 HRP antigen is suitable for ELISA and Western blots, excellent antigen for early detection of HIV seroconvertors with minimal specificity problems. 

Recombinant HIV-2 gp32, Horseradish Peroxidase LabledUsage  CHI's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.