Recombinant Mosaic Hepatitis C Virus NS4 Genotype-3

Introduction  HCV is a small 50nm, enveloped, single-stranded, positive sense RNAvirus in the family Flaviviridae.

HCV has a high rate of replication with approximately one trillion particles produced each day in an infected individual. Due to lack of proofreading by the HCV RNA polymerase, the HCV has an exceptionally high mutation rate, a factor that may help it elude the host's immune response. Hepatitis C virus is classified into six genotypes(1-6) with several subtypes within each genotype. The preponderance and distribution of HCV genotypes varies globally. Genotype is clinically important in determining potential response to interferon-based therapy and the required duration of such therapy. Genotypes 1 and 4 are less responsive to interferon-based treatment than are the other genotypes (2, 3, 5 and 6). 

Recombinant Mosaic Hepatitis C Virus NS4 Genotype-3 Description The E.coli derived recombinant protein contains the HCV NS4 immunodominant regions, amino acids 1691-1710, 1712-1733 & 1921-1940, fused with a GST tag at N-terminus. 

Method  Purified by proprietary chromatographic technique. 

Purity  Protein is >95% pure as determined by 10% PAGE

(coomassie staining). 

Formulation  1.5M urea, 25mM Tris-HCl, pH 8.0, 0.2% Triton-X & 50% glycerol. 

Recombinant Mosaic Hepatitis C Virus NS4 Genotype-3 Storage  HCV NS4 Mosaic Genotype-3 although stable at 4°C for 1 week, should be stored below -18°C.

Please prevent freeze thaw cycles. 

Specificity  Immunoreactive with sera of HCV-infected individuals. 

Applications Antigen in ELISA and Western blots, excellent antigen for detection of HCV with minimal specificity problems.