Recombinant Human Tumor Protein p53 Inducible Protein 3

Synonyms      TP53I3, PIG3, Quinone Oxidoreductase, tumor protein p53 inducible protein 3. 

Introduction TP53I3 participates in the generation of reactive oxygen species (ROS). TP53I3 has low NADPH-dependent naphtoquinone reductase activity, with a preference for 1,2-naphtoquinone over 1,4-naphtoquinone. TP53I3 has low NADPH-dependent diamine reductase activity (in vitro). TP53I3 is localized to the cytoplasm and induced in primary, non-transformed and transformed cell cultures after exposure to genotoxic agents. TP53I3 microsatellite polymorphism is associated with differential susceptibility to cancer. 

Recombinant Human Tumor Protein p53 Inducible Protein 3Description    TP53I3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 352 amino acids (1-332 a.a.) and having a molecular mass of 37.6 kDa. TP53I3 protein is fused to a 20 amino acid His tag at N-terminus and is purified by standard chromatography.

Source    Escherichia Coli. 

Physical Appearance     Sterile filtered colorless solution. 

Recombinant Human Tumor Protein p53 Inducible Protein 3Formulation   1mg/ml solution containing 20mM Tris HCl pH-8, 0.1M NaCl & 10% glycerol.

Stability     TP53I3 Human although stable at 4°C for 1 week, should be stored desiccated below -18°C. Please prevent freeze thaw cycles. 

Amino acid sequence    MGSSHHHHHH SSGLVPRGSH MLAVHFDKPG GPENLYVKEV AKPSPGEGEV LLKVAASALN RADLMQRQGQ YDPPPGASNI LGLEASGHVAELGPGCQGHW KIGDTAMALL PGGGQAQYVT VPEGLLMPIP EGLTLTQAAA IPEAWLTAFQ LLHLVGNVQA GDYVLIHAGL SGVGTAAIQLTRMAGAIPLV TAGSQKKLQM AEKLGAAAGF NYKKEDFSEA TLKFTKGAGV NLILDCIGGS YWEKNVNCLA LDGRWVLYGL MGGGDINGPLFSKLLFKRGS LITSLLRSRD NKYKQMLVNA FTEQILPHFS TEGPQRLLPV LDRIYPVTEI QEAHKYMEAN KNIGKIVLEL PQ. 

Recombinant Human Tumor Protein p53 Inducible Protein 3Purity     Greater than 90% as determined by SDS-PAGE.