Recombinant E.Coli Beta Lactamase, His Tag

Synonyms    Beta-lactamase, Cephalosporinase, ampC, ampA, b4150, JW4111.

Introduction    Beta-lactamase is a type of enzyme (EC 3.5.2.6) produced by some bacteria that is responsible for their resistance to beta-lactam antibiotics like penicillins, cephalosporins, cephamycins and carbapenems. These antibiotics have a common element in their molecular structure: a four-atom ring known as a beta-lactam. The lactamase enzyme breaks that ring open, deactivating the molecule''s antibacterial properties.

Description    Beta Lactamase is an E.coli Recombinant protein produced in E.Coli containing 379 amino acids (20-377) and having a molecular mass of 41.8kDa. Beta Lactamase is expressed with a 21 N-terminal His tag.

The LACTB is purified by proprietary chromatographic techniques.

Recombinant E.Coli Beta Lactamase, His TagSource    Escherichia Coli.

Physical Appearance    Sterile Filtered colorless solution.

Formulation    The LACTB enzyme (1 mg/ml) is supplied in 20mM Tris-HCl buffer (pH8.0) and 10% glycerol.

Recombinant E.Coli Beta Lactamase, His TagStability    LACTB although stable at 4℃ for 1 week, should be stored desiccated below -18℃. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent freeze-thaw cycles.

Amino Acid Sequence    MGSSHHHHHH SSGLVPRGSH MAPQQINDIV HRTITPLIEQ QKIPGMAVAV IYQGKPYYFT WGYADIAKKQ PVTQQTLFEL GSVSKTFTGV LGGDAIARGE IKPTTKY WPELTAKQWN GITLLHLATY TAGGLPLQVP DEVKSSSDLL RFYQNWQPAW APGTQRLYAN SSIGLFGALA VKPSGLSFEQ AMQTRVFQPL KLNHTWINVP PAEEKNYAWG YREGKAVHVS PGALDAEAYG VKSTIEDMAR WVQSNLKPLD INEKTLQQGI QLAQSRYWQT GDMYQGLGWE MLDWPVNPDS IINGSDNKIA LAARPVKAIT PPTPAVRASW VHKTGATGGF GSYVAFIPEK ELGIVMLANK NYPNPARVDA AWQILNALQ.

Recombinant E.Coli Beta Lactamase, His TagPurity    Greater than 95.0% as determined by SDS-PAGE.