Recombinant Human Acylphosphatase 1

Synonyms    Acylphosphatase-1, Acylphosphatase, erythrocyte isozyme, Acylphosphatase, organ-common type isozyme, Acylphosphate phosphohydrolase 1, ACYP1, ACYPE. 

Introduction    Erythrocyte acylphosphatase (ACYP1) is a cytosolic enzyme which catalyzes the hydrolysis of the carboxyl-phosphate bond of acylphosphates. There are two acylphophatase isoenzymes: ACYP1 and ACYP2. These isoenzymes share 60% homology and have the same substrate specificity, even though ACYP1 has a higher catalytic activity than ACYP2. 

Recombinant Human Acylphosphatase 1Description    ACYP1 Human Recombinant fused with a 23 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 122 amino acids (1-99 a.a.) and having a molecular mass of 13.6kDa. The ACYP1 is purified by proprietary chromatographic techniques. 

Source    Escherichia Coli. 

Physical Appearance    Sterile Filtered colorless solution. 

Recombinant Human Acylphosphatase 1Formulation    The ACYP1 solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1M NaCl and 1mM DTT. 

Stability    ACYP1 should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent freeze-thaw cycles. 

Purity    Greater than 90.0% as determined by SDS-PAGE. 

Recombinant Human Acylphosphatase 1Amino acid sequence    MGSSHHHHHH SSGLVPRGSH MGSMAEGNTL ISVDYEIFGK VQGVFFRKHT QAEGKKLGLV GWVQNTDRGT VQGQLQGPIS KVRHMQEWLE TRGSPKSHID KANFNNEKVI LKLDYSDFQI VK.