Recombinant Human Precursor Neurotrophin-3

Synonyms       Precursor Form Neurotrophin-3, proNT-3.

Introduction       proNT-3 (the precursor form of neurotrophin-3) functions through p75NTR and sortilin and induces neuronal apoptosis. In contrast, mature NT-3 interacts with Trk receptors and selectively promotes the survival, growth and differentiation of neurons. Since axon retrograde transport is crucial for neurotrophin signaling, proNT-3 also has an important role in the mechanism of retrograde signaling. 

Description        proNT3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 239 amino acids and having a molecular mass of 27.4kDa.

The proNT3 is purified by proprietary chromatographic techniques. 

Recombinant Human Precursor Neurotrophin-3Source       Escherichia Coli. 

Physical Appearance        Sterile Filtered White lyophilized (freeze-dried) powder. 

Formulation      proNT3 was lyophilized from a concentrated (1mg/ml) solution in 20mM PB, pH 8.0 and 300mM NaCl.

Solubility        It is recommended to reconstitute the lyophilized proNT3 in sterile 18M?-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. 

Recombinant Human Precursor Neurotrophin-3Stability        Lyophilized proNT3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution proNT3 should be stored at 4°C between 2-7 days and for future use below -18°C.

Please prevent freeze-thaw cycles. 

Purity         Greater than 95.0% as determined by:

(a) Analysis by RP-HPLC.

(b) Analysis by SDS-PAGE. 

Amino acid sequence       The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Asn-Met-Asp-Gln. 

Recombinant Human Precursor Neurotrophin-3Usage       CHI's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.