Recombinant Human Melanoma Inhibitory Activity Protein 2

Synonyms       MIA-2, MIA2, Melanoma Inhibitory Activity 2, FLJ22404. 

Introduction      MIA2 performs as a tumour suppressor in hepatocellular carcinoma. MIA2is a novel acute phase protein which its expression is found in the liver and reacts to liver damage in chronic liver diseases and is considerably higher in severe fibrosis patients . MIA2 is induced by IL6, TGF-B & and conditioned medium from activated hepatic stellate cells. MIA2 is is mainly expressed in hepatocytes. 

Recombinant Human Melanoma Inhibitory Activity Protein 2Description       MIA2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain consisting of 101 amino having a total molecular mass of 11.5 kDa.

The MIA2 is purified by proprietary chromatographic techniques. 

Source      Escherichia Coli. 

Physical Appearance        Sterile Filtered White lyophilized (freeze-dried) powder. 

Formulation      The MIA2 protein was lyophilized from a concentrated (1mg/ml) solution containing 20mM Phosphate buffer pH=7.4 and 150mM NaCl. 

Solubility       It is recommended to reconstitute the lyophilized MIA2 in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. 

Recombinant Human Melanoma Inhibitory Activity Protein 2Stability       Lyophilized MIA2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MIA2 should be stored at 4°C between 2-7 days and for future use below

-18°C.

Please prevent freeze-thaw cycles. 

Purity        Greater than 97.0% as determined by(a) Analysis by RP-HPLC.

(b) Analysis by SDS-PAGE. 

Amino acid sequence      MLESTKLLAD LKKCGDLECE ALINRVSAMR DYRGPDCRYL NFTKGEEISV YVKLAGERED LWAGSKGKEF GYFPRDAVQI EEVFISEEIQ MSTKESDFLC L. 

Recombinant Human Melanoma Inhibitory Activity Protein 2Usage        CHI's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.