Recombinant Human Macrophage Migration Inhibitor Factor, His Tag N-Terminus

Synonyms      Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GIF, MMIF, MIF. 

Introduction      The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration. 

Recombinant Human Macrophage Migration Inhibitor Factor, His Tag N-TerminusDescription       MIF human Recombinant, fused to His-tag at N-terminus, was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques.

Macrophage Inducing Factor Human Recombinant is a single, non-glycosylated, polypeptide chain having amino acids from 1-114 and having a molecular mass of 16.6 kDa. 

Source      Escherichia Coli. 

Formulation      1mg/ml solution containing PBS pH-7.4.  

Recombinant Human Macrophage Migration Inhibitor Factor, His Tag N-TerminusStability       Liquid MIF although stable 4°C for 1 week, should be stored desiccated below -18°C.

Please prevent freeze-thaw cycles. 

Purity       Greater than 95.0% as determined by(a) Analysis by RP-HPLC.

(b) Analysis by SDS-PAGE. 

Amino Acid Sequence      MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSMPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC LHSIGKIGGA QNRSYSKLLC GLLAERLRIS PDRVYINYYD MNAANVGWNN STFA. 

Recombinant Human Macrophage Migration Inhibitor Factor, His Tag N-TerminusUsage      CHI's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.