细胞剪切应力培养室平行平板流动腔细胞体外流体剪切力加载装置平行平板流体室

细胞剪切应力培养室平行平板流动腔细胞体外流体剪切力加载装置平行平板流体室

The CytoDyne Flow Chamber Systems are designed to allow cells grown on standard glass slides (38 mm by 75 mm)to be subjected to well defined laminar fluid shear stress. Fluid flow may be provided by syringe pump (short term) or by the CytoDyne Continuous Flow Loop (for long term studies).  Specifications / Features:  o Shear stress of 0-30 dyn/cm2  o All chambers come with luer connectors and are compatible with the CytoDyne continuous flow loop system.  o Vacuum seal between chamber and slide allows unit to fit on standard inverted microscope stage for real time visualization. (Vacuum pump is not provided).  Cell area 13.2 cm2 Fits 75x38mm (3"x1.5") slide Includes chamber assemble with luer fittings and gasket (medical grade silicone). Replacement Gaskets are available seperately. Useful for studies involving gene expression / Northern Blots, immunofluorescence, intravital dye and cell adhesion.

 
 

Continuous Flow Loop

The CytoDyne Continuous Flow Loop System is designed for use in conjunction with the CytoShear parallel plate flow chambers. The system provides continuous fluid flow, minimizing the required fluid volume while maintaining a sterile culture environment. The closed loop system is driven by a standard roller pump (not provided), and is ideal for both short and long term (up to 1 week) flow studies.  Specifications / Features:  o Adjustable flow rates allow shear stress of 0-30 dyn/cm2 (when used with CytoDyne flow chambers).  o Fully enclosed and autoclavable to maintain sterility o All tubing biocompatible and autoclavable  o Standard luer fittings at all connections  o Sample port for sterile sampling without disturbing the flow field  o Minimal fluid volume (as low as 12 ml) is ideal for assays of secreted products System Includes:  o Fluid Reservoir  o All tubing and connectors  o Sample port with septum

 
 
部分论文标题：
 
Shear stress-mediated NO production in inner medullary collecting duct cells
Z Cai, J Xin, DM Pollock… - American Journal of …, 2000 - Am Physiological Soc
 
Heparin inhibits the flow adhesion of sickle red blood cells to P-selectin
NM Matsui, A Varki, SH Embury - Blood, 2002 - bloodjournal.hematologylibrary.org
 
 
Fluid flow shear stress stimulates human osteoblast proliferation and differentiation through multiple interacting and competing signal transduction pathways
S Kapur, DJ Baylink, KH William Lau - Bone, 2003 – Elsevier
 
Bone cell responses to fluid flow
HY Stevens, JA Frangos - Bone Research Protocols, 2003 - Springer
 
Extracellular signal-regulated kinase-1 and-2 are both essential for the shear stress-induced human osteoblast proliferation
S Kapur, ST Chen, DJ Baylink, KHW Lau - Bone, 2004 - Elsevier
 
Extracellular signal-regulated kinase-1 and-2 are both essential for the shear stress-induced human osteoblast proliferation
S Kapur, ST Chen, DJ Baylink, KHW Lau - Bone, 2004 - Elsevier
 
Fluid shear stress synergizes with insulin-like growth factor-I (IGF-I) on osteoblast proliferation through integrin-dependent activation of IGF-I mitogenic signaling …
S Kapur, S Mohan, DJ Baylink, KHW Lau - Journal of Biological Chemistry, 2005 – ASBMB
 
Up-regulation of the Wnt, estrogen receptor, insulin-like growth factor-I, and bone morphogenetic protein pathways in C57BL/6J osteoblasts as opposed to C3H/HeJ …
KHW Lau, S Kapur, C Kesavan, DJ Baylink - Journal of Biological …, 2006 - ASBMB
 
 
Micropatterned polymer surfaces improve retention of endothelial cells exposed to flow-induced shear stress
SC Daxini, JW Nichol, AL Sieminski, G Smith… - Biorheology, 2006 - IOS Press
 
Osteoblasts and osteocytes respond differently to oscillatory and unidirectional fluid flow profiles
SM Ponik, JW Triplett… - Journal of cellular …, 2007 - Wiley Online Library
 
Fluid shear stress upregulates vascular endothelial growth factor gene expression in osteoblasts
MM Thi, DA Iacobas, S Iacobas… - Annals of the New York …, 2007 - Wiley Online Library
 
KLF2-dependent, Shear Stress-induced Expression of CD59 A NOVEL CYTOPROTECTIVE MECHANISM AGAINST COMPLEMENT-MEDIATED INJURY IN THE …
AR Kinderlerer, F Ali, M Johns, EA Lidington… - Journal of Biological …, 2008 - ASBMB
Characterization of porcine circulating progenitor cells: toward a functional endothelium
J Allen, S Khan, MC Serrano… - Tissue engineering Part …, 2008 - online.liebertpub.com
Fluid flow-induced soluble vascular endothelial growth factor isoforms regulate actin adaptation in osteoblasts
MM Thi, SO Suadicani, DC Spray - Journal of Biological Chemistry, 2010 - ASBMB
of the cytoprotective enzyme heme oxygenase-1 by statins is enhanced in vascular endothelium exposed to laminar shear stress and impaired by disturbed flow
F Ali, M Zakkar, K Karu, EA Lidington… - Journal of Biological …, 2009 - ASBMB
 
Leucocyte adhesion under haemodynamic flow conditions
C Lawson, M Rose, S Wolf - T-Cell Trafficking, 2010 – Springer
Cyclic GMP and protein kinase G control a Src-containing mechanosome in osteoblasts
H Rangaswami, R Schwappacher, N Marathe… - Science …, 2010 - stke.sciencemag.org
 
 
Fluid flow-induced soluble vascular endothelial growth factor isoforms regulate actin adaptation in osteoblasts
MM Thi, SO Suadicani, DC Spray - Journal of Biological Chemistry, 2010 - ASBMB
 
Conditional disruption of IGF-I gene in type 1α collagen-expressing cells shows an essential role of IGF-I in skeletal anabolic response to loading
C Kesavan, JE Wergedal, KHW Lau… - American Journal of …, 2011 - Am Physiological Soc
 
Pulsatile to-fro flow induces greater and sustained expression of tissue factor RNA in HUVEC than unidirectional laminar flow
R Abe, N Yamashita, A Rochier, R Abe… - American Journal of …, 2011 - Am Physiological Soc
 
Intra-ophthalmic artery chemotherapy triggers vascular toxicity through endothelial cell inflammation and leukostasis
JJ Steinle, Q Zhang, KE Thompson… - … ophthalmology & visual …, 2012 - ARVO
 
Protein kinase G and focal adhesion kinase converge on Src/Akt/β-catenin signaling module in osteoblast mechanotransduction
H Rangaswami, R Schwappacher, T Tran… - Journal of Biological …, 2012 - ASBMB
 
Effect of Frequency of Uniform and Disturbed Pulsatility Flow on Tissue Factor Expression of Human Umbilical Vein Endothelial Cells. J Vasc Med Surg 1: 110. …
T Moriguchi, BN Davis, R Abe, M Kidd, BE Sumpio - 2013 - esciencecentral.org
 
PECAM-1 phosphorylation and tissue factor expression in HUVECs exposed to uniform and disturbed pulsatile flow and chemical stimuli
T Moriguchi, BE Sumpio - Journal of vascular surgery, 2013 - Elsevier
 
Modulation of Radiation Injury Response in Retinal Endothelial Cells by Quinic Acid Derivative KZ-41 Involves p38 MAPK
JJ Toutounchian, JJ Steinle, PS Makena, CM Waters… - PloS one, 2014 - dx.plos.org