Alpha-II Spectrin Monoclonal Antibody (SIGNET)_详细资料

产品信息

靶点：来电咨询浓度： 1mg/1ml 应用范围：科研使用宿主： Goat，Rabbit，Mouse 适应物种：人，大鼠，小鼠，兔标记物：见说明书克隆性：多克隆保存条件： -20℃保存形态：液态/粉状

Alpha-II Spectrin Monoclonal Antibody (SIGNET)详细信息：

Description Monoclonal Antibody against Human Alpha-II Spectrin
(Formerly Signet Catalog Nos: 9702-02, 9702-05, 9702-10)

Intended Use **Research Use Only (RUO)**

This product is sold for laboratory research use only, not for human or in-vivo use.

Clone D8B7

Form Tissue Culture Supernatant (in PBS + 0.1% NaN3)

Host Mouse

Species Reactivity Alpha-II Spectrin Monoclonal Antibody (SIGNET)Human

IsoType igG2b

Specificity Signet’s anti-human spectrin (Clone D8B7) is developed against the alpha II spectrin SH3 domain (Xu et al., 2001). Clone D8B7 is a specific marker for human, rat and mouse alpha II spectrin or fodrin. In cerebellar cell cultures, it labels axons and periphery of cell bodies consistent with localization of fodrin (reviewed in Bennett, 1993; Goodman, 1995 & De Matteis, 2000). It does not cross-react with alpha I (erythroid) spectrin SH3 domain.

This antibody was developed against a recombinant alpha II spectrin (non-erythroid spectrin or fodrin) SH3 domain. It was subsequently purified via ammonium sulfate precipitation.

Uses This antibody is effective in immunoblotting, immunofluorescence (IF), immunohistochemistry (IHC) and ELISA.

Suggested Working Dilution The optimal working dilution should be determined for each specific assay condition.

Western blot: 1:100 - 1:1,000

IF: 1:100 - 1:500

IHC: 1:10 - 1:50 (Frozen only)

ELISA: 1:100,000- 1:1,000,000

Storage Alpha-II Spectrin Monoclonal Antibody (SIGNET)Store at -20°C or below. Upon initial thawing, apportion into working aliquots and store at -20°C or below. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. Do not store in frost-free freezers.

References Xu J, et al. Monoclonal antibodies to alphaI spectrin Src homology domain 3 associate with macropinocytic vesicles in nonerythroid cells. Brain Res 898:171-177, 2001. (WB, IF)

Xu J, et al. Human spectrin SH3 domain binding protein 1 regulates macropinocytosis in NIH 3T3 cells. J Cell Science 113:3805-3814, 2000.

De Matteis, MA, Morrow JS. Spectrin tethers and mesh in the biosynthetic pathway.J. Cell Science 113:2331-2343, 2000.

Swanson JA, Watts C. Macropinosytosis. Trends Cell Biol 5:424-427, 1995.

Goodman SR, et al. Brain spectrin: of mice and men. Brain Res Bull 36:593-606, 1995.

Lamaze C, Schimid SL. The emergence of clathrin-independent pinocytic pathways.Curr Opin Cell Biol 7:573-580, 1995.

Bennett V, Gilligan DM. The spectrin-based membrane skeleton and micron scale organization of the plasma membrane. Annu Rev Cell Biol 9:27-66, 1993.

Warranty/Conditions Covance products may not be resold or modified for resale without prior written approval.

Alpha-II Spectrin Monoclonal Antibody (SIGNET)

xy-7003R   PMPCB线粒体导肽水解酶β抗体
xy-9408R   PRR15富含脯氨酸蛋白15抗体
xy-10486R   protein C light chain维生素K依赖的蛋白C轻链抗体
xy-10487R   protein C Activation peptide
xy-0686R   PTEN一种肿瘤YZ基因抗体（C端）
xy-1478R   PAFR血小板活化因子受体抗体
xy-5422R   PP1C gamma蛋白磷酸酶γ1抗体
xy-4181R   p53R2核苷酸还原酶M2B抗体
xy-2384R   PDE4A磷酸二酯酶4A抗体
xy-3643R   PLASTIN3丝束蛋白T Plastin抗体
xy-3638R   Plexin A2神经丛蛋白A2抗体
xy-3640R   Plectin网蛋白抗体
xy-10171R   PTOV1前列腺肿瘤高表达蛋白1抗体
xy-5596R   phospho-P53(Thr55)磷酸化肿瘤YZ基因P53抗体
xy-4180R   P2Y2嘌呤ATP受体抗体
xy-2210R   phospho-p38 MAPK (Thr180 + Tyr182)磷酸化丝裂原活化蛋白激酶p38抗体
xy-2219R   Pectinesterase inhibitor 18果胶酶YZ蛋白18抗体
xy-3614R   PPAR alphaα型-过氧化酶活化增生受体抗体（PPAR α, PPAR-α）
xy-2561R   PSGD/HOXB13同源盒蛋白HOXB13抗体
xy-5537R   Phospho-PDPK1(Ser393)磷酸化3磷酸肌醇依赖性蛋白激酶1抗体
xy-3716R   Phospho-p63 (Ser160+Ser162)磷酸化P63肿瘤YZ基因抗体
xy-2270R   Phospho-PAK4(Ser99)磷酸化p21激活激酶4抗体
xy-3717R   Phospho-p73(Tyr99)磷酸化P73肿瘤YZ基因抗体
xy-3718R   Phospho-p95 NBS1 (Ser343)磷酸化DNA修复蛋白NBS1抗体
xy-1573R   PUMAp53正向细胞凋亡调控因子抗体
xy-4151R   PLDL1磷脂酶C1样蛋白抗体
xy-6742R   Prostate and colon associated protein前列腺癌和结肠癌相关蛋白
xy-0361R   Protein G重组蛋白G抗体
xy-2067R   PIK3CA磷脂酰肌醇激酶催化亚单位A抗体
xy-1476R   PDI蛋白质二硫键异构酶抗体
xy-1345R   PODXL足细胞特异蛋白抗体
xy-2090R   P53(wt-p53)肿瘤YZ基因抗体（野生型P53）
xy-2413R   PAX7配对盒基因7抗体
xy-0655R   PAK5激活激酶PAK7抗体

您可能感兴趣的产品

Alpha-II Spectrin Monoclonal Antibody (SIGNET)

产品信息

联系我们

关注我们

Description		Monoclonal Antibody against Human Alpha-II Spectrin (Formerly Signet Catalog Nos: 9702-02, 9702-05, 9702-10)
Intended Use		Research Use Only (RUO) This product is sold for laboratory research use only, not for human or in-vivo use.
Clone		D8B7
Form		Tissue Culture Supernatant (in PBS + 0.1% NaN3)
Host		Mouse
Species Reactivity		Alpha-II Spectrin Monoclonal Antibody (SIGNET)Human
IsoType		igG2b
Specificity		Signet’s anti-human spectrin (Clone D8B7) is developed against the alpha II spectrin SH3 domain (Xu et al., 2001). Clone D8B7 is a specific marker for human, rat and mouse alpha II spectrin or fodrin. In cerebellar cell cultures, it labels axons and periphery of cell bodies consistent with localization of fodrin (reviewed in Bennett, 1993; Goodman, 1995 & De Matteis, 2000). It does not cross-react with alpha I (erythroid) spectrin SH3 domain. This antibody was developed against a recombinant alpha II spectrin (non-erythroid spectrin or fodrin) SH3 domain. It was subsequently purified via ammonium sulfate precipitation.
Uses		This antibody is effective in immunoblotting, immunofluorescence (IF), immunohistochemistry (IHC) and ELISA.
Suggested Working Dilution		The optimal working dilution should be determined for each specific assay condition. Western blot: 1:100 - 1:1,000 IF: 1:100 - 1:500 IHC: 1:10 - 1:50 (Frozen only) ELISA: 1:100,000- 1:1,000,000
Storage		Alpha-II Spectrin Monoclonal Antibody (SIGNET)Store at -20°C or below. Upon initial thawing, apportion into working aliquots and store at -20°C or below. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. Do not store in frost-free freezers.
References		Xu J, et al. Monoclonal antibodies to alphaI spectrin Src homology domain 3 associate with macropinocytic vesicles in nonerythroid cells. Brain Res 898:171-177, 2001. (WB, IF) Xu J, et al. Human spectrin SH3 domain binding protein 1 regulates macropinocytosis in NIH 3T3 cells. J Cell Science 113:3805-3814, 2000. De Matteis, MA, Morrow JS. Spectrin tethers and mesh in the biosynthetic pathway.J. Cell Science 113:2331-2343, 2000. Swanson JA, Watts C. Macropinosytosis. Trends Cell Biol 5:424-427, 1995. Goodman SR, et al. Brain spectrin: of mice and men. Brain Res Bull 36:593-606, 1995. Lamaze C, Schimid SL. The emergence of clathrin-independent pinocytic pathways.Curr Opin Cell Biol 7:573-580, 1995. Bennett V, Gilligan DM. The spectrin-based membrane skeleton and micron scale organization of the plasma membrane. Annu Rev Cell Biol 9:27-66, 1993.
Warranty/Conditions		Covance products may not be resold or modified for resale without prior written approval.