Description |  | Purified Polyclonal Antibody against alpha 1-Antichymotrypsin (ACT) (Formerly Signet Catalog Nos: 250-01, 250-16 L1 Predilute, 250-26 L2 Predilute) |
Intended Use |  | ** In Vitro Diagnostic (IVD) ** |
Form |  | Purified Antibody (in 0.01M PBS + 0.1% NaN3 + 1% BSA) |
Host |  | Rabbit |
Species Reactivity |  | Alpha 1-Antichymotrypsin Polyclonal Antibody, Purified (SIGNET)Human |
Specificity |  | The alpha-1-antichymotrypsin (ACT) polyclonal antibody detects human ACT and does not cross react with ACT from mouse, rat, cow, chicken, dog, goat, guinea pig, horse, swine or sheep. The ACT polyclonal antibody labels the monocytes and macrophages of human tonsil, skin, and colon. There is no cross reactivity with connective tissue or other cells. This antibody was generated using human alpha-1-antichymotrypsin. Concentrated Format: SIG-3250-1000 [1 mL] Prediluted Formats: SIG-3250-16 [6 mL] SIG-3250-26 [6 mL] is ready-to-use with Biotin-based detection systems such as Signet’s USA Ultrastreptavidin Detection (SIG-32250). |
Uses |  | Alpha 1-Antichymotrypsin Polyclonal Antibody, Purified (SIGNET)This antibody is effective in immunohistochemistry (IHC). |
Suggested Working Dilution |  | The optimal working dilution should be determined for each specific assay condition. - IHC: ≥1:40 (concentrated format) with Biotin based detection systems such as Signet’s USA Ultrastreptavidin Detection (SIG-32250).
Tissue Sections: Formalin-fixed, paraffin-embedded tissues Pretreatment: Not required Incubation: 20-60 minutes at room temperature |
Notes |  | Positive tissue (human): Tonsil |
Storage |  | Store between 2-8°C. |
References |  | Meis JM, et al. A comparative marker study of large cell lymphoma, Hodgkin’s disease, and true histiocytic lymphoma in paraffin-embedded tissue. Am J Clin Pathol 86:591-599, 1986. Nathrath WBJ, et al. Lysozyme (muramidase) and alpha-1-anti-chymotrypsin as immunohistochemical tumour markers. Acta Histochem 25:69-72; 1982. |
Warranty/Conditions |  | Covance products may not be resold or modified for resale without prior written approval. |